Flow Cytometry

项目总结（见说明）： 核心D -流式细胞术研究核心提供了技术专长和主要仪器的集中资源，以支持和加强艾滋病毒发病机制和治疗中需要使用流式细胞术表型分析，细胞分选或特定免疫细胞功能评估的基础和转化研究的实验设计和执行。一个主要目标是提供科学环境和物质资源，使初级研究人员能够将流式细胞术技术应用于他们的项目，并鼓励已建立的研究人员启动创新的试点研究。核心人员包括：主任，Celsa Spina，PhD，D（ABMLI）;实验室经理/高级技术专家，拥有10年以上使用各种细胞仪的经验（T. Rambaldo）;两名技术专家，每人都有20年以上的经验（M。O'Keefe，N. Sekiya）;和一个初级技术员，有4年的经验（D。Sirypangno）。主要仪器包括：三台细胞分析仪（2-激光FACS Canto I，3-激光FACS Canto II，2-激光iCyte LSC）和两台高速细胞分选仪（3-激光FACS Aria II，4-激光MoFlo XDP），其中一台位于BSL-2/3生物防护设施内。 核心基金支持的艾滋病毒/艾滋病研究范围涵盖病毒调节和发病机制的基础工作（AI 038201、AI 081668、AI 095623、AI 096113、DK 035108、GM 032373）用于药物、疫苗和基因疗法的临床前开发（AI 033292、AI 055332、AI 071803、AI 076558、AI 079031）用于病毒传播的临床研究（AI 074621），病毒潜伏期（AI 080193，AI 096113），神经生物学（DA 026306、MH 083552、MH 097520）以及对实验处理的免疫和病毒应答（AI 064086、AI 087164、MH 085610）。支持和促进上述每一领域的研究：通过核心主任和技术支持人员提供关于实验设计和数据解释的专家咨询;选择最先进的仪器进行补充功能;通过专门的技术操作员使用设备;通过既定的补充机制提供所有服务;数据分析是根据各个研究项目的具体目标定制的。核心与研究人员合作，开发和应用实现资助项目研究目标所需的新方法，并培训初级研究人员最佳和正确地使用流式细胞术方法来解决他们的研究假设。此外，该中心还提供了一个独特的生物隔离设施，对艾滋病毒感染和其他生物危害性样本进行活细胞分选，满足了圣地亚哥艾滋病毒研究界的特殊需求。

PROJECT SUMMARY (See instructions): Core D - The Flow Cytometry Research Core provides a centralized resource of technical expertise and major instrumentation to support and enhance the experimental design and execution of basic and translational research in HIV pathogenesis and treatment that require use of flow cytometric phenotypic analysis, cell sorting, or evaluation of specific immune cell functions. A prime goal is to provide the scientific environment and the material resources to enable junior investigators to apply flow cytometry technology to their projects and to encourage established investigators to initiate innovative pilot studies. Core personnel include: the Director, Celsa Spina, PhD, D(ABMLI); a lab manager/senior technologist, with >10 yrs. experience using a broad range of cytometry instruments (T. Rambaldo); two technical specialists, each with >20 yrs. experience (M. O'Keefe, N. Sekiya); and a junior technician, with 4 yrs. experience (D. Sirypangno). Major instrumentation includes: three cell analyzers (2-laser FACS Canto I, 3-laser FACS Canto II, 2-laser iCyte LSC) and two high-speed cell sorters (3-laser FACS Aria II, 4-laser MoFlo XDP), one of which is housed within a BSL-2/3 biocontainment facility. The spectrum of HIV/AIDS research, supported by the Core, spans basic work on viral regulation and mechanisms of pathogenesis (AI038201, AI081668, AI095623, AI096113, DK035108, GM032373) to preclinical development of drugs, vaccines, and gene therapies (AI033292, AI055332, AI071803, AI076558, AI079031) to clinical studies of viral transmission (AI074621), viral latency (AI080193, AI096113), neurobiology (DA026306, MH083552, MH097520), and immune and viral responses to experimental treatments (AI064086, AI087164, MH085610). To support and promote research in each of these areas: expert consultation on experimental design and data interpretation is provided through the Core Director and technical support staff; state-of-the-art instrumentation is selected for complementary functions; equipment use is accessible through dedicated technician operators; all services are provided through an established recharge mechanism; and data analysis is customized to the specific objectives of individual research projects. The Core works with investigators to develop and apply new methods that are needed to attain the research goals of funded projects, and trains junior investigators to optimally and correctly use flow cytometry approaches to address their research hypotheses. In addition, the Core fills a special need of the San Diego HIV research community by providing a unique biocontainment facility that performs live cell sorting of HIV-infected and other biohazardous samples.