Development of laser spectroscopic methods for quantification of 14C

描述（由申请人提供）：我们建议开发一种桌面大小的光谱方法，以定量毫克大小的生物样品中的14C，其水平低至天然同位素丰度，使用一种称为腔环down光谱的技术。该系统的购买和操作成本将大大降低，最终可能取代目前最先进的加速器质谱分析技术。该方法将为14C分析提供一个新的范式，允许对14C标记的治疗实体的代谢、处置、命运和质量平衡进行研究，用于药物开发，用于风险评估的潜在毒物，以及用于个性化医疗的生物标志物分析。考虑到预期的成本和操作的简单性，这种新技术将比目前可用的任何生物医学或生物化学实验室都能负担得起操作这种仪器，从而使更高灵敏度的14C分析成为可能。

DESCRIPTION (provided by applicant): We propose to develop a table-top sized spectroscopic method to quantify 14C in milligram sized biological samples at levels down to the natural isotopic abundance, using a technique called Cavity Ring Down Spectroscopy. This system will be much cheaper to acquire and to operate, and could eventually replace the current state of the art technology -- accelerator mass spectrometry. This approach will lead to a new paradigm for 14C analysis in allowing studies to be carried out on the metabolism, disposition, fate and mass balance of 14C-labeled therapeutic entities for drug development, potential toxicants for risk assessment, and analysis of biomarkers for personalized medicine. Given the expected cost and simplicity of operation, this new technique will enable much greater access to high sensitivity 14C analysis than now currently available as any biomedical or biochemistry laboratory could afford to operate such an instrument.