Total Synthesis of Alsmaphorazine A, an Alkaloid Natural Product Inhibitor of Nit

描述（由申请人提供）：一氧化氮合酶在呼吸系统、心血管系统、神经系统和免疫系统的生命过程的正常运作中起着至关重要的作用，最近的进展表明，酶功能不正常与癌症、中风和神经退行性疾病的发生有关。异构体选择性一氧化氮合酶抑制剂已被证明可以调节这些酶的活性，并有助于治疗许多人类疾病。Alsmaphorazine A是一种从Alstonia pneumatophora中分离的单萜吲哚生物碱，在脂酶刺激的LPS刺激的J774.1巨噬细胞中显示出有希望的诱导型一氧化氮合酶抑制作用（IC 50 =49.2 μ M），其抑制作用呈剂量依赖性，而不影响细胞活力。自发现以来，没有通过全合成制备阿司马福拉嗪家族的成员，因此合成这些化合物和非天然类似物将使其能够作为一氧化氮合酶抑制剂或其他疾病的潜在治疗剂进行评估。 该提案描述了一种简明的方法，从简单的起始材料的独特的六环核心的alsmaporazine A的全合成，并概述了合成路线的扩展到alsmaporazine B和许多合成衍生物。该方法采用分子内Diels-Alder、分子内或分子间Heck和（1，3）-偶极环加成来确保具有1，2-恶嗪烷、异恶唑烷和吲哚啉部分的笼状杂环框架。这种灵活的合成路线能够快速评估关键转化和不对称合成或制备非天然生物碱类似物的简易修饰。

DESCRIPTION (provided by applicant): Nitric oxide synthase plays an essential role in the proper functioning of vital processes in respiratory, cardiovascular, nervous, and immune systems, and recent advances have shown that improper enzyme function has been linked to the onset of cancer, stroke, and neurodegenerative diseases. Isoform-selective nitric oxide synthase inhibitors have been shown to modulate the activity of these enzymes and help treat numerous human diseases. Alsmaphorazine A, a monoterpene indole alkaloid isolated in small quantities from Alstonia pneumatophora, has shown promising inducible nitric oxide synthase inhibition in lipase stimulated LPS-stimulated J774.1 macrophage cells (IC50=49.2 ¿M) in a dose-dependent manner without affecting cell viability. No members of the alsmaphorazine family have been prepared by total synthesis since their discovery, so synthetic access to these compounds and unnatural analogs would enable their evaluation as nitric oxide synthase inhibitors or potential therapeutics for other diseases. This proposal describes a concise approach to the total synthesis of the unique hexacyclic core of alsmaphorazine A from simple starting materials and outlines the extension of the synthetic route to alsmaphorazine B and numerous synthetic derivatives. The approach employs an intramolecular Diels-Alder, intra- or intermolecular Heck, and (1,3)-dipolar cycloaddition to secure the caged heterocyclic framework which bears 1,2-oxazinane, isoxazolidine, and indoleneine moieties. This flexible synthetic route enables rapid evaluation of key transformations and facile modification for asymmetric synthesis or preparation of unnatural alkaloid analogs.