CELLULAR IMAGING, SURGERY, AND TISSUE PROCESSING CORE FACILITY

项目概述（见说明书）：核心B是支持显微成像、定量分析和周围神经损伤模型在PPG中使用的必要条件。Core被这三个项目广泛使用，并直接有助于实现这些项目的多个目标。核心B的范围和资源已通过增加或更新新仪器（例如，由PPG的行政补充资助的用于电子显微镜的AMT数码相机）大大扩大，以满足PPG项目的需要。一个重要的补充是提供专业知识和技术帮助，以提供所有3个项目都使用的啮齿动物周围神经损伤的单一模型。这消除了手术技术和特定肌肉或神经中微量注射示踪剂项目之间的差异，并促进了项目之间数据的直接比较，以帮助解释。核心设施由经验丰富的调查员和研究管理员指导，由三名训练有素的合格研究人员协助。核心为PPG小组成员提供所有必要的培训，以获得授权访问和使用核心仪器和设施；PPG成员有权优先使用该设施。服务包括对仪器和分析技术的使用进行培训和指导，协助设计和/或解释实验，按要求动手进行组织处理和成像，电子显微镜处理和成像，显微外科手术和示踪剂注射。重要的资源包括3台共聚焦显微镜（一台是带有电生理装置的多光子仪器），电子显微镜套件，神经细胞系统，荧光解剖显微镜，低温恒温器，振动原子和超显微切片机，低温替代系统，设备齐全的组织学实验室，最先进的啮齿动物显微外科套件，以及四个用于图像处理和数据分析的离线工作站。数据安全存储，每个PPG PI都可以安全地访问PPG共享数据云（1.0 tb），在那里他们可以访问自己和彼此的数据，并与核心中的数据采集仪器进行无缝通信。

PROJECT SUMMARY (See Instructions): Core B is essential to support the microscopic imaging, quantitative analysis, and peripheral nerve injury model used in this PPG. The Core is used extensively by all three projects and contributes directly to multiple goals of these projects. The scope and resources of Core B have been significantly expanded by addition or update of new instrumentation (e.g. an AMT digital camera for the electron microscope, funded by an Administrative Supplement to the PPG) to meet the needs of the PPG projects. A significant addition is the provision of expertise and technical help to provide a single model of peripheral nerve injury in rodents that is used by all 3 projects. This eliminates variability between projects in surgical technique and microinjection of tracers in specific muscles or nerves, and facilitates direct comparison of data between projects to aid in interpretation. The Core facility is directed by a highly experienced investigator and research administrator, assisted by three highly trained and qualified research associates. The Core provides all necessary training to members of the PPG groups for authorized access and use of Core instruments and facilities; PPG members have priority access to the facility. Services include training and guidance for use of instruments and analytical techniques, assistance with design and/or interpretation of experiments, hands-on performance of tissue processing and imaging as required, electron microscopy processing and imaging, and microsurgery and tracer injection. Significant resources include 3 confocal microscopes (one is a multiphoton instrument with electrophysiology setup), electron microscope suite, Neurolucida system, fluorescence dissecting microscope, cryostats, vibratomes and ultramicrotomes, cryosubstitution system, a fully equipped histology laboratory, access to a state-of-the-art suite for microsurgery in rodents, and four off-line workstations for image processing and data analysis. Data is securely stored, and each PPG PI has secure access to a PPG shared data Cloud (1.0 terabyte) where they can access their own and each other's data and communicate seamlessly with the data acquisition instruments in the Core.