Mechanisms of Eukaryotic Gene Regulation

 描述(申请人提供)：Kadonaga实验室研究的长期目标是了解双侧(两侧对称动物)中RNA聚合酶II转录的调节机制。为什么研究基因表达如此重要？对基因表达的透彻理解是至关重要的，因为一系列生物现象(包括许多人类疾病，如一些癌症)的基础可以追溯到一个或一组基因的适当或不适当的表达。因此，对基因表达机制的新见解将对广泛的生物学研究产生广泛的影响。Kadonaga实验室一直在寻求探索染色质和转录的新领域，这些领域往往被低估。通过这种方式，他们已经能够为理解基因调控做出了许多独特的贡献。在未来，他们计划研究基础转录过程的多样性机制，以及染色质在基因表达调控中的作用，特别是非核小体组蛋白结构。这些主题将被单独研究，也将被联合研究，以最好地了解基因是如何在双侧动物，如果蝇和人类中调节的。

 DESCRIPTION (provided by applicant): The long-term objective of the research in the Kadonaga laboratory is to understand the mechanisms of regulation of RNA polymerase II transcription in bilateria (bilaterally-symmetric animals). Why is it important to study gene expression? A thorough understanding of gene expression is essential because the basis of a diverse range of biological phenomena (including many human diseases such as some cancers) can be traced to the proper or improper expression of a gene or set of genes. Thus, new insights into the mechanisms of gene expression will have a broad impact upon a wide range of biological research. The Kadonaga lab has sought to explore new and often underappreciated frontiers in chromatin and transcription. In this manner, they have been able to make a number of unique contributions to the understanding of gene regulation. In the future, they plan to study the diversity of mechanisms of the basal transcription process and the role of chromatin, particularly non-nucleosomal histone-containing structures, in the regulation of gene expression. These topics will be studied both individually as well as in concert to gain the best possible understanding of how genes are regulated in bilateria such as Drosophila and humans.