Analytical and Translational Genomics Shared Resource

分析和翻译基因组学共享资源 摘要 UNCC分析和翻译基因组学（基因组学）共享资源（原UNCC凯克- UNM基因组学资源）通过提供具有成本效益的，全面的 服务于下一代测序（NGS）和数据分析、微阵列、实时PCR和其他基因组学- 向联合国赔偿委员会成员和非成员提供有关技术，包括病人样本的基因组分析 用户.学院院长斯科特·尼斯博士监督由全职员工运营的资源，包括 经验丰富的湿实验室研究技术人员加上一个团队进行初步的生物信息学和数据分析。的 联合国赔偿委员会研究行政处负责管理该资源。在上一个供资期间， 经历了一个重大转变，从主要提供基于微阵列的检测到新的重点是NGS 服务包括靶向（例如基因组）测序和外显子组测序以及RNA-seq和ChIP- 使用其Ion Torrent和Ion Proton测序仪器进行测序。该资源保持一个专用的小型- 这将有助于建立一个集群，用于存储大型数据集，并促进对NGS数据的综合生物信息学分析。的 下一代测序服务是对现有服务的补充， 使用Affyesterday仪器和定量实时PCR测定。资源执行初始处理 利用先进的统计工具和软件，为用户详细分析微阵列和NGS数据 包装和坚持严格的质量控制准则，并与生物统计学共享资源 和生物信息学和高维数据分析共享资源，为用户提供一个完整的 从详细的实验设计到先进的生物信息学和统计数据， 分析.通过一个集中的在线系统跟踪预订、计费和使用情况。资源 主任和工作人员与联合国赔偿委员会的研究方案密切合作，制定新的方法， 创新的基础和转化研究目标和资源积极促进和传播 通过维护最新的网站，了解新的基因组学技术和数据分析技术 页，组织每月会议，重点是翻译基因组学和生物信息学，并给予 在联合国赔偿委员会会议和务虚会上作介绍。在上一个5年项目期间，33名联合国赔偿委员会成员 来自3个联合国赔偿委员会研究方案的200名研究人员使用了该资源，共产生了32份出版物，其中4份是 目前正在等待PMCID。在2013年7月至2014年6月的报告年度， 占总资源使用量的88%，并得到109个同行评审赠款的支持。

ANALYTICAL & TRANSLATIONAL GENOMICS SHARED RESOURCE ABSTRACT The UNMCC Analytical and Translational Genomics (Genomics) Shared Resource (formerly the UNMCC Keck- UNM Genomics Resource) supports basic and translational cancer research by providing cost-effective, full service next-generation sequencing (NGS) and data analysis, microarray, real-time PCR and other genomics- related technologies, including the genomic analysis of patient samples, to UNMCC members and non-member users. Faculty Director Scott Ness, Ph.D. supervises the Resource, which is operated by a full time staff including experienced wet lab research technicians plus a team performing initial bioinformatics and data analysis. The UNMCC Research Administration manages the Resource. During the previous funding period the Resource underwent a major transition from primarily providing microarray-based assays to a new emphasis on NGS services including targeted (e.g. gene panel) sequencing and exome sequencing as well as RNA-seq and ChIP- seq using its Ion Torrent and Ion Proton sequencing instruments. The Resource maintains a dedicated mini- cluster for storage of large data sets and to promote the integrated bioinformatics analyses of NGS data. The next generation sequencing services complement the existing services provided through its established Affymetrix instruments and with quantitative real-time PCR assays. The Resource performs initial processing and detailed analysis of microarray and NGS data for users, utilizing sophisticated statistical tools and software packages and adhering to strict quality control guidelines, and interacts with the Biostatistics Shared Resource and the Bioinformatics and High Dimensional Data Analysis Shared Resource to provide users with a complete range of services from detailed experimental design through advanced bioinformatics and statistical data analysis. Reservations, billing and usage are tracked through a centralized on-line system. The Resource Director and staff work closely with UNMCC research programs to develop new methodologies that promote innovative basic and translational research goals and the Resource actively facilitates and disseminates information about new genomics technologies and data analysis techniques by maintaining an up-to-date web page, organizing monthly meetings focused on translational genomics and bioinformatics and by giving presentations at UNMCC meetings and retreats. During the previous 5-yr project period, 33 UNMCC members from 3 UNMCC Research Programs used the Resource, resulting in a total of 32 publications, of which 4 are presently pending PMCIDs. In the reporting year of July 2013 – June 2014, UNMCC members were responsible for 88% of total Resource usage and were supported by 109 peer-reviewed grants.