Neurolucida BrainMaker Imaging System

 描述（由申请人提供）：冷泉港实验室的多名NIH支持的神经科学家组成了一个高度整合的社区，研究啮齿动物神经回路的发育和功能以及神经精神疾病的模型。我们整合了多种方法和技术，从小鼠基因工程到最先进的神经成像，电生理学和行为分析。神经回路研究的一个关键组成部分是在细胞和亚细胞分辨率和大脑体积下可视化，重建和量化神经元形态和连接。半自动荧光显微镜结合Neurolucida系列重建，定量和显示已被证明是一个有效的系统。我们目前的工作站是在12年前购买的，功能和容量非常有限。我们正在申请资金从MBF Bioscience购买Neurolucida BrainMaker成像系统，用于细胞分辨率全脑成像和重建。这将使我们能够继续进行尖端的神经回路研究，并支持多个NIH资助的研究人员。

 DESCRIPTION (provided by applicant): Multiple NIH supported neuroscientists at Cold Spring Harbor Laboratory form a highly integrated community studying rodent neural circuit development and function and models of neuropsychiatric disorders. We integrate multiple approaches and techniques from mouse genetic engineering to state of the art neuroimaging, electrophysiology, and behavior analysis. A key component of neural circuit study is to visualize, reconstruct, and quantify neuronal morphology and connectivity at cellular and subcellular resolution and in large brain volume. Semi-automated fluorescence microscopy combined with Neurolucida serial reconstruction, quantification, and display has proven to be an effective system. Our current work station was acquired over 12 years ago, with very limited functionality and capacity. We are requesting funds to purchase a Neurolucida BrainMaker Imaging System from MBF Bioscience for cell resolution whole brain imaging and reconstruction. This will allow us to continue to carry out cutting edge neural circuit research and support multiple NIH funded researchers.