CryoEM Data Collection Facility Consortium at NCMI

 描述（由申请人提供）：贝勒医学院的国家高分子成像中心（NCMI）是主办实验室，提供部分时间在其JEM 3200 FSC和JEM 2200 FS电子显微镜上进行冷冻电子显微镜（cryoEM）和断层扫描（cryoET）数据采集设施联盟。这两种仪器都配备了场发射枪和柱内能量过滤器，而JEM 2200 FS还具有Zernike相位板附件。这两种仪器目前都配备了以积分模式运行的DE 20和DE 12相机。这些显微镜已被有效地用于各种生物标本，包括大分子，分子机器和细胞，在国家的最先进的分辨率。我们的设施将分配给他建议的财团，同时考虑到正在进行的研究在东道国机构。我们的首席PI Wah Chiu是推动cryoEM和cryoET超越当前界限的公认专家，并在领导几个NIH资助的研究中心和学术培训项目方面拥有数十年的经验。美国有11个参与机构，由在cryoEM和/或cryoET方面有研究记录的研究人员领导。日常操作将由兼职cryoEM/ET科学家和IT人员进行。远程用户将使用亲自和远程数据收集协议。联合会将为远程用户提供旅费。我们要求拨款购置以点算模式运作的直接检测仪器，以应付各类样本的需要。主办机构会提供一名行政助理，以支援使用者探访及各项联盟活动，并会提供等额拨款，以购买DDD及足够的硬盘作短期数据储存之用。参与的PI将参与制定联合体治理的具体政策和开发培训活动。第一年的时间安排将在参与者之间均匀分配，并根据标本准备情况具有一定的灵活性。显微镜时间和优先级的分配将在第二年及以后根据所有PI任命的外部显微镜分配委员会的建议进行分配，以避免利益冲突。Al PI将每季度在WebEx上开会讨论技术和管理问题，我们将举行年度用户会议。一个咨询委员会将对联合体业务的所有方面和用户的数据结果进行年度审查，以确保拟议设施的最高生产力和使用率。参与机构的成员资格将由所有主要研究者根据外部咨询机构的意见进行动态审查 在我们工作的第二年。

 DESCRIPTION (provided by applicant): The National Center for Macromolecular Imaging (NCMI) at Baylor College of Medicine is the host laboratory offering to provide a portion of the time on their JEM3200FSC and JEM2200FS electron microscopes for a cryo-electron microscopy (cryoEM) and tomography (cryoET) data acquisition facility Consortium. Both instruments are equipped with a field emission gun and an in-column energy filter, while the JEM2200FS also has a Zernike phase plate attachment. Both instruments are currently equipped with DE20 and DE12 cameras operated in integrating mode. These microscopes have been used productively for a variety of biological specimens, including macromolecules, molecular machines and cells, at state-of-the-art resolution. Our facilities will be allocated for he proposed Consortium while taking into account the ongoing research in the host institution. Our lead PI, Wah Chiu is a recognized expert in pushing cryoEM and cryoET beyond current boundaries and has decades of experience in leading several NIH funded research centers and academic training programs. There are 11 participating institutions across the USA, led by investigators who have a research track record in cryoEM and/or cryoET. Day-to-day operation will be carried out by a part-time cryoEM/ET scientist and IT staff. Both in person and remote data collection protocols will be used for distant users. The Consortium will provide travel funds for the distant users. We request funding for a Direct Detection Device (DDD) operated in counting mode to meet the needs of all types of specimens. The host Institution will provide an administrative assistant to support user visits and various Consortium activities, and matching funds to purchase the DDD and adequate hard drives for short-term data storage. The participating PIs will be involved in formulating the specific policies for Consortium governance and developing training activities. Scheduling will be allocated evenly in the first year among participants with some flexibility based on specimen readiness. Allocation of microscope times and priorities will be assigned in the second year and beyond based on recommendations of an external microscope allocation committee appointed by all the PIs to avoid conflict of interest. Al PIs will meet quarterly on WebEx to discuss technical and administrative issues, and we will hold an annual user meeting. An annual review of all aspects of the Consortium operation and the data outcomes of the users will be conducted by an advisory committee to assure the highest productivity and usage of the proposed facility. The membership of the participating institutions will be dynamically reviewed by all the PIs with the inputs from the external advisory committee after the second year of our operation.