Core A Islet Production

摘要/摘要 核心A：小岛制作核心。胰岛功能和完整性是血糖动态平衡的关键环节。 小岛生产核心支持DRC调查人员的研究，他们分析了 从小鼠和人的胰腺分离的胰岛的遗传或药物干预。加州大学旧金山分校 刚果民主共和国也有非常强大的胰岛免疫和移植计划。孤立的小岛对于 旨在对抗胰岛自身免疫和最大限度减少移植排斥反应的研究。 目的：刚果民主共和国小岛生产核心巩固、增强和传播专业知识和资源 为目前正在研究或计划研究胰岛细胞生物学的刚果民主共和国调查人员提供胰岛准备， 胰岛功能和/或移植。核心提供以下功能： 1.胰岛纯化。用于胰岛生物学的人和小鼠胰岛的加工和制备， 自身免疫和移植研究。 2.胰岛功能分析。胰岛素产生和其他的实时生物和生化分析 人和小鼠胰岛在不同条件下的功能特征和胰岛活性 评估用于移植研究的胰岛准备质量。 对刚果民主共和国社区的好处：刚果民主共和国调查人员不需要投入时间和资源来高度确定这一点 他们实验室内的专业能力。不同DRC所使用的均一胰岛制备方法 研究人员还可以进行跨研究比较，从而在研究之间产生意想不到的联系。35 目前，每年直接费用总计6,580,009美元的不同项目正受益于这一核心。 仅在过去的两年中，就总共准备了807次小鼠胰岛(每次准备1到20只小鼠)用于 12个刚果民主共和国研究实验室。另外，对另外3名研究人员进行了45只小鼠胰岛制备 当他们考虑他们的研究是否适用于糖尿病研究时。加州大学旧金山分校还有一家领先的 临床胰岛移植计划。为人类胰岛移植开发的设施已经 由刚果民主共和国利用，使人类胰岛可用于研究。在过去的一年，人类研究 为6名刚果民主共和国调查人员的研究准备了小岛。根据设施的容量和可用性 在人类研究级胰腺中，我们建议提供这种独特的人类胰岛能力 加州大学旧金山分校以外的调查人员(见地区核心)。 技术发展：人类和模式生物的独特能力促进了翻译 加州大学旧金山分校和整个地区的核心用户进行的研究。刚果民主共和国也有强大的计划，资金 通过刚果民主共和国赠款，成功地改进了对胰岛准备情况的监测 和功能。这使核心的活动不仅使翻译和基础研究受益，而且 以及临床试验和参与试验的患者。

Abstract/Summary Core A: Islet Production Core. Islet function and integrity represent a critical node in glucose homeostasis. The Islet Production Core supports research by DRC investigators who analyze the functional responses to genetic or pharmacologic interventions in islets isolated from pancreases of mice and humans. The UCSF DRC also has a very strong islet immunology and transplantation program. Isolated islets are essential for studies oriented towards counteracting islet autoimmunity and minimizing transplant rejection. Purpose: The DRC Islet Production Core consolidates, enhances and disseminates expertise and resources in islet preparation to DRC investigators who currently study or have plans to study pancreatic islet cell biology, islet function and/or transplantation. The Core provides the following capabilities: 1. Islet Purification. Processing and preparation of human and mouse islets for use in islet biology, autoimmunity and transplantation studies. 2. Islet Functional Analysis. Real time biological and biochemical analysis of insulin production and other functional aspects of human and mouse islets to characterize islet activity under different conditions and to assess islet preparation quality for transplantation studies. Benefits to DRC Community: DRC investigators need not commit time and resources to establishing this highly specialized capability within their laboratory. The uniform islet preparation methods used by different DRC investigators also can enable cross-study comparisons to generate unanticipated linkages between studies. 35 different projects totaling $6,580,009 annual direct costs currently are benefiting from this Core. In the past 2 years alone, mouse islets were prepared a total of 807 times (from 1 to 20 mice per prep) for 12 DRC research laboratories. A further 45 mouse islet preparations were conducted for 3 other investigators as they considered whether their studies had applications to diabetes research. UCSF also has a leading clinical islet transplantation program. The facilities developed for those human islet transplants have been leveraged by the DRC for making human islets available for research studies. In the last year, human research islets were prepared for studies by six DRC investigators. With the capacity of the facilities and the availability of human research-grade pancreases, we propose to provide this unique human islets capability to investigators beyond UCSF (see Regional Core). Technology Development: Unique capabilities in human and model organisms facilitate translational research by Core users at UCSF and throughout the region. The DRC also has robust programs, funded through the DRC grant, that have successfully developed improvements in the monitoring of islet preparation and function. This enables the activities of the Core to benefit not only translational and basic research, but also clinical trials and the patients participating in them.