Microscopy and Flow Cytometry

项目摘要 显微镜核心和流式细胞术（核心B）将提供与标准相关的专业知识和服务 电子显微镜（EM），以及荧光显微镜和流式细胞术，根据需要，由每个 项目的三个组成部分。这样一个标本制备和成像的集中设施将是成本- 有效和促进更有序和有效地使用EM和荧光显微镜资源， 及时完成计划项目。所要求的预算是适度的50%的工资， 显微镜/成像实验室经理以及研究技术员和EM的50%服务合同 和荧光显微镜。一个新的增加是24%的时间为流式细胞术专家。的具体职能 核心将包括：（1）电子显微镜标本制备;（2）病毒、重组病毒、 颗粒及其与-碳水化合物和抗体的复合物，重构脂质囊泡以评估纯度 和浓度;（3）来自杯状病毒感染的动物和人的粪便样品的成像;（4）来自杯状病毒感染的动物和人的粪便样品的成像。 病毒感染细胞的染色薄切片;（5）免疫电子显微镜;（6）数据采集、共享，以及 存档;（7）调度和日常EM维护;（8）荧光检测的标本制备 （9）流式细胞术，以获得关于病毒感染的细胞和关于从人外周血单个核细胞收集的PBMC的定量数据。 诺如病毒感染者（10）显微镜和流程的安排和日常维护 细胞计数器。核心总监和副总监将协调显微镜和流程的所有方面 细胞计数核心。

PROJECT SUMMARY The Microscopy Core and Flow Cytometry (Core B) will provide expertise and service related to standard electron microscopy (EM), as well as fluorescent microscopy and flow cytometry as needed by each of the three project components. Such a centralized facility for specimen preparation and imaging will be cost- effective and facilitate more orderly and efficient use of the EM and fluorescent microscope resources to complete the proposed projects in a timely fashion. The requested budget is modest with a 50% salary for a microscopy/imaging lab manager and as well as a research technician and 50% service contract for the EM and fluorescent microscopes. A new addition is 24% time for a flow cytometry expert. The specific functions of the core will include: (1) specimen preparation for electron microscopy; (2) imaging of viruses, recombinant particles, and their complexes with –carbohydrate and antibodies, reconstituted lipid vesicles to evaluate purity and concentration; (3) imaging of fecal samples from calicivirus infected animals and humans; (4) imaging of stained thin sections of virus infected cells; (5) immuno-electron microscopy; (6) data acquisition, sharing, and archiving; (7) scheduling and day-to-day EM maintenance; (8) specimen preparation for fluorescent microscopy; (9) flow cytometry to obtain quantitative data on virus-infected cells and on PBMCs collected from norovirus-infected individuals (10) scheduling and day-to-day maintenance of the microscopes and flow cytometers. The Core Directors and Associate Director will coordinate all aspects of the Microscopy and Flow Cytometry Core.