Shared Resource: Cellular Imaging

细胞成像共享资源 项目摘要/摘要 细胞成像共享资源(CISR)为联盟成员提供了一套全面的 先进的高分辨率成像技术、定量凝胶和斑点成像以及定量图像 癌症研究的分析。该资源包括两个以服务为基础的实验室，电子 显微镜(EM)和科学成像(SI)。EM实验室提供样品准备、访问 显微切割、冷冻和免疫电磁技术，以及关于传输和扫描电磁以及原子的培训 力显微镜。SI实验室提供用于共焦的先进成像系统， 反褶积、多光子、时间推移、全内反射荧光、高含量分析、整张幻灯片 扫描和其他光学显微镜技术，以及用于可视化和定量的强大软件 显微镜数据分析。随着研究需求和优先事项的发展，CI已扩展到新的领域，以 为更广泛的调查人员和研究项目提供支持。新服务的示例包括： 高含量分析和平板筛选；全载玻片扫描和分析；以及冷冻和免疫-EM。 总体而言，所有联盟研究计划目前都在使用资源，这两个组成部分都提供了基本的 为会员研究提供服务和技术。该资源不断评估新技术和 他们可以给财团带来的好处，并通过课堂积极推动此类技术的使用， 与个别研究人员进行研讨会、研讨会和一对一讨论。

Cellular Imaging Shared Resource Project Summary/Abstract The Cellular Imaging Shared Resource (CISR) provides Consortium members with a comprehensive set of advanced high resolution imaging techniques, quantitative gel and blot imaging, and quantitative image analysis for cancer research. The resource is comprised of two service-based laboratories, Electron Microscopy (EM) and Scientific Imaging (SI). The EM laboratory offers sample preparation, access to microtomy, cryo- and immuno- EM technologies, and training in transmission and scanning EM, and atomic force microscopy. The SI laboratory provides access to advanced imaging systems for confocal, deconvolution, multi photon, time lapse, total internal reflection fluorescence, high content analysis, whole slide scanning, and other light microscopy techniques, along with powerful software for visualization and quantitative analysis of microscopy data. As research needs and priorities evolve, CI has expanded into new areas to provide support for a broader range of investigator and research programs. Examples of new services include: high content analysis and plate screening; whole slide scanning and analysis; and Cryo and Immuno-EM. Overall, all Consortium research programs currently use the resource and both components provide essential services and technologies for member research. The resource constantly evaluates new technologies and the benefits they can bring to the Consortium, and actively promotes the use of such technologies through classes, workshops, seminars, and one-on-one discussions with individual researchers.