Preclinical Imaging & Microscopy (IPIM)

项目摘要/摘要：辐照、临床前成像和显微镜（IPIM）共享资源 辐照共享资源是NCCC CCSG的一个组成部分，已有30多年的历史。为 目前提交的IPIM已扩展到包括临床前成像、光学细胞（共聚焦） 和电子显微镜。这一扩展得到了NCI CCSG补充赠款的促进 （3 P30 CA 023108 - 32 S4），支持新的小动物成像设施的翻新。施工 于2012年完成。新的共享资源由六个NCCC研究中的四个研究人员使用 项目包括癌症机制、分子治疗学、癌症成像与放射生物学，以及 免疫学和癌症免疫治疗。IPIM结合了基于动物和细胞的电离辐射服务， 先进的细胞和电子显微镜成像，以及大型和小型动物成像能力， 包括9.4T小口径磁共振成像（MRI）、小口径/啮齿动物 正电子发射断层扫描（mPET），小口径/啮齿动物计算机断层扫描（mCT），高分辨率x- 体内小口径CT、整个动物（啮齿动物）生物发光（IVUS）和荧光（红外）成像。 此外，IPIM监督大型动物成像仪器，包括MRI，CT， 荧光透视/血管造影和超声;所有这些都是许多类型的临床前转化研究所必需的。 IPIM密切参与最近完成的外科中心的开发和使用， 创新（CSI），一种独特的新型术中临床和临床前手术成像设施，包括 最先进的MRI和CT扫描仪，能够在外科手术过程中扫描患者。最后 IPIM（Strawbridge，Hoopes）负责监督和促进几个交变磁场（AMF）发生器 和线圈目前用于激活磁性纳米粒子在正在进行的NCCC纳米技术癌症 研究计划。IPIM活动由P. Jack Hoopes（DVM，PhD）和高级内部人员监督。 在这些服务的所有科学方面具有非常重要的知识和经验的咨询委员会。 活动由七名经验丰富、训练有素的工作人员集中安排和管理 科学家和资源管理者。每个部门都接受主任的监督， 在这一特定领域训练有素的主管。大卫J.格莱斯顿（DSc，DABMP），辐射物理学主任， 达特茅斯希区柯克医疗中心，指导辐照服务。一位研究兽医凯伦博士 穆迪（DVM，MS），指导临床前和动物成像资源。Radu Stan（医学博士，博士）指导 光蜂窝（包括共焦）服务。电子显微镜设备和程序由Dr. Charles Daghlian（PhD）.由于需要日常专业知识和支持，辐照资源和 光学细胞（共聚焦）和电子显微镜资源每个都有来自 经理伦德尔·斯特劳布里奇、克里斯托弗·奥戈莫和肯尼斯·奥恩多夫，沿着负责图像处理 顾问亚历克斯·哈托夫，所有在现场协助和访问信息的人。

Project Summary/Abstract: Irradiation, Preclinical Imaging, & Microscopy (IPIM) Shared Resource The Irradiation Shared Resource has been a component of the NCCC CCSG for more than 30 years. For the current submission, IPIM has been expanded to include preclinical imaging, optical cellular (confocal) and electron microscopy. This expansion has been facilitated by an NCI CCSG supplemental grant (3P30CA023108-32S4) that supported renovation of a new small animal imaging facility. Construction was completed in 2012. The new Shared Resource is used by investigators from four of the six NCCC research programs, including Cancer Mechanisms, Molecular Therapeutics, Cancer Imaging & Radiobiology, and Immunology & Cancer Immunotherapy. IPIM combines animal and cell-based ionizing irradiation services, sophisticated cell and electron microscopic imaging, and large and small animal imaging capabilities, including 9.4T small-bore magnetic resonance Imaging (MRI), small bore/rodent positron emission tomography (mPET), small bore / rodent computed tomography (mCT), high resolution x- vivo small bore CT, whole animal (rodent) bioluminescent (IVUS) and fluorescence (Infrared) imaging. Additionally, IPIM oversees large-animal imaging instrumentation, including MRI, CT, fluoroscopy/angiography, and ultrasound; all necessary for many types of preclinical translational research. IPIM is involved intimately in the development and use of the recently completed Center for Surgical Innovation (CSI), a unique novel intraoperative clinical and preclinical surgery-imaging facility that includes state-of-the-art MRI and CT scanners that enable scanning a patient during surgical procedures. Finally the IPIM (Strawbridge, Hoopes) oversees and facilitates several alternating magnetic field (AMF) generators and coils currently used to activate magnetic nanoparticles in the ongoing NCCC nanotechnology cancer research program. IPIM activities are overseen by P. Jack Hoopes (DVM, PhD) and a senior internal advisory board with very significant knowledge and experience in all scientific aspects of these services. Activities are scheduled and managed by a centralized staff of seven highly experienced and trained scientists and resource managers. Each service receives oversight from the director as well as a co- director highly trained in that specific area. David J. Gladstone (DSc, DABMP), chief of radiation physics, Dartmouth-Hitchcock Medical Center, directs the irradiation service. A research veterinarian, Dr. Karen Moodie (DVM, MS), directs the preclinical and animal imaging resource. Radu Stan (MD, PhD) directs the optical cellular (including confocal) services. The Electron Microscopy facility and program is directed by Dr. Charles Daghlian (PhD). Due to the need for daily expertise and support, the irradiation resource and optical cellular (confocal) and electron microscopy resources each have operational support from managers Rendall Strawbridge, Christopher Ogomo, and Kenneth Orndorff, along with image processing consultant Alex Hartov, all who are on site to assist and access information.