Critical resources provided by UNMC RAP biorepository stimulate cancer research

项目摘要 高度创新的快速尸检胰腺(RAP)计划解决了胰腺的一个主要限制 癌症研究：缺乏代表所有疾病阶段的高质量的胰腺癌人类标本。 我们的计划是快速收集大量在死亡一小时内启动的所有器官和液体 并在五个小时内完成，是世界上独一无二的计划。从这些文件中生成存储库 尸检可以捕获死亡时存在的完整病史，并最终允许 用于收集来自癌前病变、原发肿瘤的全谱疾病的样本 和转移性疾病。RAP计划是内布拉斯加州大学医学中心的核心组成部分 (UNMC)胰腺癌研究计划，任务是收集、归档和分发 来自最近死于胰腺癌患者的组织和体液。该股的单位主任 UNMC弗雷德·巴菲特和帕梅拉·巴菲特癌症中心的胰腺癌研究项目是迈克尔博士 A.霍林斯沃斯。霍林斯沃斯博士领导下的研究助理教授保罗·格兰杰奈特博士说， 是说唱项目的负责人，也是这项研究专家奖的申请者。说唱节目 在UNMC的胰腺癌症研究计划内开展工作，对 部分资金来自：胰腺癌孢子P50赠款；早期检测组织正在进行的和即将获得的U01赠款 研究网络；作为肿瘤微环境网络一部分的U54赠款；以及几笔R01赠款 个人调查员。Grangenett博士的一个关键角色是与合作者一起确定样本 研究项目所需要的，根据需要修改我们的SOP以获得这些样本，然后 将这些样本提供给调查人员。对于这一资源的领导层来说，必须是一名训练有素的 具有丰富经验的研究专家通过参与尸检，全面 对可用的患者样本的了解，以及在科学水平上与每个患者进行互动的能力 调查员。收集的样本包括原发肿瘤、残留的未受影响的胰腺、远处转移等 未受影响的组织以及血液和腹水等液体。这些组织和体液被迅速采集 根据旨在维持组织细胞和分子完整性的SOP。精选的新鲜样品有 立即处理或用于活细胞培养/异种移植程序。这些程序在中确定 这两个项目都是根据赔偿委员会和其他机构的供资项目的需要而制定的。目前，我们 将新鲜样本运送到其他进行血液生物标记物研究的实验室(蛋白质、RNA、 DNA)、循环中的肿瘤细胞、细胞培养(包括有机物)和其他过程。剩下的样本 被冻结，保存在长期存储设施中，并通过请求和协作提供 地方、国家和国际胰腺癌研究。Grangenett博士在所有方面都发挥着重要作用 这些生物化石的收集、加工、注释和分布。

Project Summary The highly innovative Rapid Autopsy Pancreas (RAP) program addresses a major limitation in pancreatic cancer research: the lack of quality human specimens for pancreatic cancer representing all stages of disease. Our program of rapid collection of large quantities of all organs and fluids that initiates within an hour of death and is completed within five hours is a unique program in the world. The repository generated from these autopsies allows for the capture of the complete disease history present at the time death and ultimately allows for collection of samples representing the full spectrum of disease from premalignant lesions, primary tumor and metastatic disease. The RAP program is a core component of the University of Nebraska Medical Center (UNMC) Pancreatic Cancer Research program that is tasked with the collection, archiving and distribution of tissues and fluids from patients that have recently passed with pancreatic cancer. The Unit Director of the Pancreatic Cancer Research program at the Fred and Pamela Buffett Cancer Center of UNMC is Dr. Michael A. Hollingsworth. Dr. Paul Grandgenett, a Research Assistant Professor under the aegis of Dr. Hollingsworth's, is the director of the RAP program and the applicant for this Research Specialist Award. The RAP program operates within the Pancreatic Cancer Research Program at UNMC, and is important to and currently funded in part by: a pancreatic cancer SPORE p50 grant; ongoing and pending U01 grants from the Early Detection Research Network; a U54 grant as part of the Tumor Microenvironment Network; and several R01 grants to individual investigators. A key role of Dr. Grandgenett is to work with collaborators to determine the samples that are needed for a research project, to modify our SOP as needed to obtain those samples, and then to provide those samples to investigators. It is essential for the leadership of this resource to be a highly trained Research Specialist with extensive experience through participation in the autopsies, a comprehensive knowledge of the patient samples available, as well as the ability to interact on a scientific level with each investigator. Specimens collected include primary tumor, residual unaffected pancreas, distant metastases, all unaffected tissues, as well as fluids such as blood and ascites. These tissues and fluids are rapidly harvested under an SOP that aims to maintain cellular and molecular integrity of the tissue. Selected fresh samples are processed immediately or used for live cell culture/xenograft procedures. These procedures are determined in advance and are based on the needs of funded projects both at UNMC and at other institutions. Currently, we ship fresh samples to other laboratories conducting research on blood based biomarkers (proteins, RNA, DNA), circulating tumor cells, cell cultures including organoids, and other processes. The remaining samples are frozen, maintained in long-term storage facilities and made available through request and collaboration for local, national and international pancreatic cancer studies. Dr. Grandgenett has a major role in all aspects of the collection, processing, annotation and distribution of these biospecimens.