Animal Care Core

核心B：动物护理核心 摘要(核心B) 动物护理核心(核心B)为动物护理和住房、进口、活体提供项目支持 MMPC-UCD的程序性取样和动物模型的建立。核心通过以下方式实现这一点 在最小延迟和减少检疫的情况下引进和驯化小鼠。核心可以访问和 监督一个专门的体内表型鉴定设施和相关的最先进的，包括一个高水平的 遏制“经批准的供应商地位”障碍隔膜。此外，核心拥有对新的、 UCD灵生小鼠研究中心(GMRC)。核心提供大量的科学支持服务 同样，包括重新衍生和创造转基因突变小鼠，重新制作各种 在KOMP或MMRRC存储库中超低温保存的模型。这些新的和现有的鼠标模型可以 然后进行繁殖以产生用于体内和体外表型的雄性和雌性队列，包括重新派生 进入GMRC进行肠道微生物组研究。Core还运营着一个显微外科套件，用于创建 手术操作的小鼠模型，如减肥手术模型。酷睿可以为 研究人员对接受垂直袖状胃切除术(VSG)和Roux en Y胃切除术的小鼠进行了队列研究 搭桥(RYGB)减肥手术。根据用户的请求，核心可以与其他表型一起工作 提供这些模型的测试、操作和采样的核心。此外，酷睿还可以将这些 手术操作的模型以及任何基因工程小鼠品系要求调查人员 来自我们批准的供应商屏障隔膜，便于直接进入受助机构的隔膜。这 确保研究人员在他们的机构接收和使用这些动物模型时的最小延误。这个 CORE还提供辅助服务和程序，包括体内葡萄糖测试的血液样本 耐受性测试和胰岛素耐受性测试，然后将其发送到表型鉴定核心(例如，核心C， 内分泌学和新陈代谢核心)进行分析。核心还可以提供遥测植入和 留置导尿管用于连续采血。核心由两个训练有素的实验室提供支持 动物兽医、三名训练有素的啮齿动物外科技术人员、一名进出口协调员和两名 表型鉴定技术人员。在这些方面，动物关怀核心是唯一有资格支持各种不同的 MMPC-UCD使用者的VIVO需求以满足他们的特定需求，从快速进口和检测，从基因上- 基因工程和基因组编辑(Cas9)小鼠品系，减肥和其他手术操作的模型， 在屏障条件下的专门住房，用于直接运送到调查人员，并在 微生物组操作的灵知生菌条件。

CORE B: ANIMAL CARE CORE ABSTRACT (CORE B) The Animal Care Core (Core B) provides project support for animal care and housing, importation, in vivo procedural sampling, and animal model creation for the MMPC-UCD. The Core accomplishes this through importation and acclimation of mice with minimal delay and reduced quarantine. The Core has access to and oversight of a dedicated in vivo Phenotyping Facility and associated state-of-the-art vivaria, including a high- containment “approved vendor-status” barrier vivarium. In addition, the Core has primary access to a new, UCD Gnotobiotic Mouse Research Center (GMRC). The Core provides numerous scientific support services as well, including rederivation and creation of genetically-modified mutant mice, re-animation of a variety of models cryopreserved within the KOMP or MMRRC repositories. These new and extant mouse models can then be bred to produce male and female cohorts for in vivo and in vitro phenotyping, including rederivation into the GMRC for gut microbiome studies. The Core also operates a Microsurgery Suite for creating surgically-manipulated mouse models, such as bariatric surgical models. The Core can produce for researchers cohorts of mice that have undergone Vertical Sleeve Gastrectomy (VSG) and Roux en Y Gastric Bypass (RYGB) bariatric procedures. Upon request from users, the Core can work with the other Phenotyping Cores to provide testing, manipulation, and sampling of these models. In addition, the Core can ship these surgically-manipulated models as well as any genetically-engineered mouse lines to requesting investigators from our approved vendor barrier vivarium, facilitating direct entry into the recipient institution's vivarium. This ensures minimal delay for investigators for receipt and use of these animal models at their institution. The Core also provides ancillary services and procedures, including blood sampling for in vivo testing for glucose tolerance tests and insulin tolerance tests, which are then sent to the Phenotyping Cores (e.g., Core C, Endocrinology and Metabolism Core) for analysis. The Core can also provide telemetry implantation and indwelling catheter placement for continuous blood sampling. The Core is supported by two trained laboratory animal veterinarians, three trained rodent surgical technicians, one import/export coordinator, and two phenotyping technicians. In these ways, the Animal Care Core is uniquely qualified to support the varied in vivo needs of MMPC-UCD users to meet their specific needs, from rapid importation and testing, genetically- engineered and genome-edited (Cas9) mouse lines, bariatric and other surgically-manipulated models, specialized housing under barrier conditions for direct shipping to investigators, and maintenance under gnotobiotic conditions for microbiome manipulation.