Bioprinting Patterning for Cell-Laden Constructs

TR&D 2：细胞负载结构的生物打印图案化 项目摘要 组织和器官工程的一个主要挑战是生产三维（3D）仿生， 具有临床相关尺寸、形状和结构完整性的细胞组织构建体， 受损或受伤的组织。为了满足这一需求，我们一直在研究3D生物打印技术， 被设计用于打印载有细胞的水凝胶生物墨水以及聚合物生物材料，以制造复杂的， 模拟天然组织结构的多细胞活组织构建体。此外，我们一直在 优化生物材料的配方，以作为3D生物打印的支架，并提供 将细胞和生物材料成功递送到体内特定位置所需的生物环境 3D结构。已经变得明显的是，载有细胞的生物材料的图案化设计对于细胞的形成是至关重要的。 实现充足的营养和功能结果。在这个项目中，我们将（1）开发生物墨水配方， 功能化以控制具有高可印刷性的细胞定位，（2）制造生物功能生物墨水制剂， 控制细胞微环境，以及（3）验证生物打印的多个细胞群体与相互作用 用于体内骨组织再生的功能。该项目的成果-将与广泛的 社区-将建立理想的参数，需要细胞定位在3D打印组织， 从而建立了用于工程化组织的社区范围的方法。

TR&D 2: Bioprinting Patterning for Cell-Laden Constructs Project Summary A major challenge for tissue and organ engineering is the production of three-dimensional (3D) biomimetic, cellular tissue constructs of clinically relevant size, shape, and structural integrity needed for the replacement of damaged or injured tissues. To address this need, we have been investigating 3D bioprinting technologies, which are designed to print cell-laden hydrogel bioinks as well as polymeric biomaterials, to manufacture complex, multi-cellular living tissue constructs that mimic the structure of native tissues. In addition, we have been optimizing the formulation of biomaterials to serve as the scaffolding for 3D bioprinting, and providing the biological environment needed for the successful delivery of cells and biomaterials to specific locations within the 3D structures. It has become evident that the patterning design of the cell-laden biomaterials is critical for achieving adequate nutrition and functional outcomes. In this project, we will (1) develop bioink formulations and functionalize to control cell positioning with high printability, (2) fabricate biofunctional bioink formulations to control the cell microenvironment, and (3) validate bioprinted multiple cell populations with interacting functionalities for bone tissue regeneration in vivo. The results of this project - which will be shared with the broad community - will be to establish the ideal parameters needed for cell positioning during 3D printing of tissues, thus establishing a community wide approach for engineering tissues.