Preclinical Imaging & Microscopy (IPIM)

项目摘要/摘要：辐射、临床前成像和显微镜(IPIM)共享资源 30多年来，辐射共享资源一直是NCCC CCSG的一个组成部分。为 目前提交的IPIM已扩展到包括临床前成像、光学细胞(共焦) 和电子显微镜。这一扩展得到了NCI CCSG补充拨款的推动 (3P30CA023108-32S4)，支持翻新一个新的小动物成像设施。建筑业是 于2012年完工。新的共享资源由来自六项NCCC研究中的四项的调查人员使用 课程，包括癌症机制、分子治疗、癌症成像和放射生物学，以及 免疫学与癌症免疫治疗。IPIM结合了基于动物和细胞的电离辐射服务， 复杂的细胞和电子显微镜成像，以及大小动物的成像能力， 包括9.4T小口径磁共振成像(MRI)、小口径/啮齿动物 正电子发射断层扫描(MPET)，小口径/啮齿动物计算机断层扫描(MCT)，高分辨率x-射线扫描 活体小口径CT、全动物(啮齿动物)生物发光(IVUS)和荧光(红外)成像。 此外，IPIM还监督大型动物成像仪器，包括MRI、CT、 透视/血管造影术和超声波；所有这些都是许多类型的临床前转化性研究所必需的。 IPIM密切参与最近建成的外科中心的开发和使用 创新(CSI)，一种独特的术中临床和临床前手术-成像设施，包括 最先进的核磁共振和CT扫描仪，可以在手术过程中扫描患者。最后是 IPIM(稻草桥，胡普斯)监督和促进几个交流磁场(AMF)发电机 和线圈，目前用于激活正在进行的NCCC纳米技术癌症中的磁性纳米颗粒 研究计划。IPIM活动由P.Jack Hoope(DVM，PhD)和一名高级内部人员监督 咨询委员会在这些服务的所有科学方面都有非常丰富的知识和经验。 活动由七名经验丰富和训练有素的中央工作人员安排和管理 科学家和资源经理。每个部门都要接受主管的监督，以及一名协管人 在那个特定领域受过高度训练的导演。大卫·J·格拉德斯通(DSC，DABMP)，辐射物理学主任， 达特茅斯-希区柯克医疗中心负责指导放射服务。一位研究兽医，凯伦博士 Moodie(DVM，MS)，指导临床前和动物成像资源。拉杜·斯坦(医学博士)执导 光蜂窝(包括共焦)服务。电子显微镜设备和程序由Dr。 查尔斯·达格利安(博士)。由于需要日常专业知识和支持，辐射资源和 光学细胞(共焦)和电子显微镜资源都有来自 经理Rendall Strawbridge，Christopher Ogomo和Kenneth Orndorff，以及图像处理 顾问亚历克斯·哈托夫，所有人都在现场协助和获取信息。

Project Summary/Abstract: Irradiation, Preclinical Imaging, & Microscopy (IPIM) Shared Resource The Irradiation Shared Resource has been a component of the NCCC CCSG for more than 30 years. For the current submission, IPIM has been expanded to include preclinical imaging, optical cellular (confocal) and electron microscopy. This expansion has been facilitated by an NCI CCSG supplemental grant (3P30CA023108-32S4) that supported renovation of a new small animal imaging facility. Construction was completed in 2012. The new Shared Resource is used by investigators from four of the six NCCC research programs, including Cancer Mechanisms, Molecular Therapeutics, Cancer Imaging & Radiobiology, and Immunology & Cancer Immunotherapy. IPIM combines animal and cell-based ionizing irradiation services, sophisticated cell and electron microscopic imaging, and large and small animal imaging capabilities, including 9.4T small-bore magnetic resonance Imaging (MRI), small bore/rodent positron emission tomography (mPET), small bore / rodent computed tomography (mCT), high resolution x- vivo small bore CT, whole animal (rodent) bioluminescent (IVUS) and fluorescence (Infrared) imaging. Additionally, IPIM oversees large-animal imaging instrumentation, including MRI, CT, fluoroscopy/angiography, and ultrasound; all necessary for many types of preclinical translational research. IPIM is involved intimately in the development and use of the recently completed Center for Surgical Innovation (CSI), a unique novel intraoperative clinical and preclinical surgery-imaging facility that includes state-of-the-art MRI and CT scanners that enable scanning a patient during surgical procedures. Finally the IPIM (Strawbridge, Hoopes) oversees and facilitates several alternating magnetic field (AMF) generators and coils currently used to activate magnetic nanoparticles in the ongoing NCCC nanotechnology cancer research program. IPIM activities are overseen by P. Jack Hoopes (DVM, PhD) and a senior internal advisory board with very significant knowledge and experience in all scientific aspects of these services. Activities are scheduled and managed by a centralized staff of seven highly experienced and trained scientists and resource managers. Each service receives oversight from the director as well as a co- director highly trained in that specific area. David J. Gladstone (DSc, DABMP), chief of radiation physics, Dartmouth-Hitchcock Medical Center, directs the irradiation service. A research veterinarian, Dr. Karen Moodie (DVM, MS), directs the preclinical and animal imaging resource. Radu Stan (MD, PhD) directs the optical cellular (including confocal) services. The Electron Microscopy facility and program is directed by Dr. Charles Daghlian (PhD). Due to the need for daily expertise and support, the irradiation resource and optical cellular (confocal) and electron microscopy resources each have operational support from managers Rendall Strawbridge, Christopher Ogomo, and Kenneth Orndorff, along with image processing consultant Alex Hartov, all who are on site to assist and access information.