Experimental and Computational Genomics

摘要 实验和计算基因组学核心（ECGC）是一个新创建的核心，它集成了所有 Sidney Kimmel综合癌症中心（SKCCC）的癌症基因组学服务。主 核心的使命是让SKCCC研究人员利用正在进行的癌症基因组学革命， 加速基础发现和转化研究。ECGC简化了服务， 以前通过三个独立的核心提供：微阵列核心，下一代测序核心和 生物信息学核心。新的联合结构认识到，前三个核心的活动 高度相互依赖，并正式确立了协调计算资源、招聘 和教育活动，为SKCCC的共同利益。新设立的中央机构 简化了对实验和计算基因组学技术和专业知识的获取， 在实验和计算方面的教学和实践教育的机会， 癌症基因组学庞大的实验室和分析专业知识汇集在新整合的 跨学科的核心，现在可在一个单一的和明确界定的核心，所有SKCCC调查。的 这个核心所提供的服务是其他地方所没有的。SKCCC成员寻求外部资源 需要与多个供应商单独交谈，只能获得零碎的服务。 该核心由Leslie科普博士领导; Sarah Wheelan医学博士博士学位;和Srinavasan Yegnasubramanian， 医学博士，哲学博士、他们汇集了基因组学技术、计算生物学 和生物统计学/生物信息学。核心提供获得和教育，国家的最先进的，下一个- 代测序、微阵列技术和用于探测基因组改变的分析工作流程 （包括SNV、插入缺失和结构改变）、癌症表观基因组学（例如，ChIP-seq和DNA甲基化组）， 转录组学和宏基因组学对于每个项目，核心使用多学科诊所模型来建立 执行实验和分析计划，首先与每个研究人员会面，然后帮助他们 通过他们基因组学实验的各个方面，包括实验设计，高通量微阵列， 或下一代测序工作流程，以及计算/生物信息学数据分析。另夕h 核心提供广泛的教育资源，包括短期课程，讲习班和研讨会，以便 调查人员可以获得充分理解和利用所产生的数据和分析所需的技能。的 该核心的成功体现在几乎所有计划的高水平使用和引用的支持上 在大量有影响力的出版物、国家/国际会议摘要和成功的赠款中， 应用. SKCCC托管核心 报告期：2015年1月1日至2015年12月31日

ABSTRACT The Experimental and Computational Genomics Core (ECGC) is a newly created Core that integrates all of the cancer genomics services in the Sidney Kimmel Comprehensive Cancer Center (SKCCC). The primary mission of the Core is to allow SKCCC investigators to harness the ongoing revolution in cancer genomics to accelerate their basic discovery and translational research. The ECGC has streamlined services that were previously offered through three separate Cores: the Microarray Core, Next-Generation Sequencing Core and Bioinformatics Core. The new joint structure recognizes that the activities of the previous three Cores are highly interdependent and formalizes a long-standing commitment to coordinate computing resources, hiring and educational activities for the common good of the SKCCC. The newly created centralized structure has streamlined access to experimental and computational genomics technologies and expertise, and enhanced opportunities for didactic and hands-on education in both the experimental and computational aspects of cancer genomics. The immense laboratory and analytical expertise brought together in the newly integrated cross-disciplinary Core are now available, in a single and clearly defined Core, to all SKCCC investigators. The services provided by this Core are not available elsewhere. An SKCCC member looking to outside resources would need to independently talk with multiple vendors and would only be able to obtain piecemeal services. This Core is led by Leslie Cope, Ph.D.; Sarah Wheelan, M.D., Ph.D.; and Srinavasan Yegnasubramanian, M.D., Ph.D., who bring together multidisciplinary expertise in genomics technologies, computational biology and biostatistics/bioinformatics. The Core provides access to, and education in, state-of-the-art, next- generation sequencing, microarray technologies and analytical workflows for probing genomic alterations (including SNVs, indels and structural alterations), cancer epigenomics (e.g., ChIP-seq and DNA methylome), transcriptomics and metagenomics. For each project, the Core uses a multidisciplinary clinic model to establish and execute an experimental and analytical plan, first meeting with each investigator and then helping them through all aspects of their genomics experiments, including experimental design, high-throughput microarray or next-generation sequencing workflows, and computational/bioinformatics data analysis. Additionally, the Core provides extensive educational resources, including short courses, workshops and a symposium, so that investigators can gain the skills they need to fully understand and utilize the data and analyses generated. The success of this Core is evidenced by the high level of usage across nearly all Programs and the cited support in a large number of impactful publications, national/international conference abstracts and successful grant applications. SKCCC Managed Core Reporting Period: Jan. 1, 2015, to Dec. 31, 2015