Octet Red384

项目总结/摘要 我们申请资金购买ForteBio Octet Red 384仪器来测量蛋白质-蛋白质相互作用。到 最大限度地提高研究效率，升级后的仪器将被放置在俄勒冈州健康与科学 大学蛋白质组学共享资源。唯一能测量蛋白质-蛋白质结合速率和亲和力的仪器 OHSU现有的相互作用提供了非常低的通量，需要新的仪器来增加研究 我们机构的生产力。新仪器将用于研究几种疾病的原因和治疗方法。 疾病，包括精神疾病，艾滋病毒，听力损失和贫血，以及研究治疗方法 这些疾病。纯化的蛋白质将通过将一个配偶体结合到廉价和可移植的生物传感器上进行分析 探针，然后使用生物层干涉测量法以高灵敏度测量第二蛋白质的结合。八位位组 Red 384每天可以有效地测量许多完整的相互作用，每个涉及6-8个蛋白质 浓度并产生kon和koff以及KD。该文书的采用将履行 OHSU的研究人员越来越需要将蛋白质化学中最先进的工具纳入他们的研究中。 research.该仪器所提供的能力将加快发现的进展，并提高 检测和治疗人类疾病。

Project Summary/Abstract We request funds to purchase a ForteBio Octet Red384 instrument to measure protein-protein interactions. To maximize research productivity, the upgraded instrument will be placed in the Oregon Health & Science University Proteomics Shared Resource. The only instruments that measure rates and affinities of protein-protein interactions available at OHSU provide very low throughput, and new instrument is required to increase research productivity at our institution. The new instrument will be used to study the cause and treatment of several diseases including psychiatric disorders, HIV, hearing loss, and anemia, as well as to study methods for treating those diseases. Purified proteins will be analyzed by binding one partner to cheap and dispensable biosensor probes, then using biolayer interferometry to measure binding of a second protein with high sensitivity. The Octet Red384 can efficiently measure many complete sets of interactions per day, each involving 6-8 protein concentrations and producing both kon and koff as well as KD. The introduction of this instrument will fulfill the growing needs of the researchers at the OHSU to incorporate state-of-the-art tools in protein chemistry into their research. The capability afforded by this instrument will speed the progress of discovery and improve the detection and treatment of human disease.