Proteomics Shared Resource

CORE-013：蛋白质组学共享资源（PSR） 项目总结/摘要 OSUCCC蛋白质组学共享资源（PSR）为CCSG研究人员提供了先进的质量 质谱（MS）仪器，用于样品制备的辅助仪器和专家工作人员， 蛋白质组学研究，包括数据分析。两位质谱学终身教授专家， Michael Freitas博士（MBCG）和Vicki Wysocki博士担任PSR主任和高级教师 顾问，分别。他们在癌症蛋白质组学方面的专业知识为PSR提供科学领导， 蛋白质化学、生物信息学和质谱方法开发。PSR提供了许多 蛋白质组学服务的用户，属于蛋白质鉴定，表征， 量化能够可靠地识别蛋白质是PSR的核心作用。蛋白质的例子 特征分析包括：鉴定翻译后修饰，替代剪接形式， 新蛋白质测序和蛋白质-蛋白质相互作用分析。PSR的具体目标是：1） 提供先进的基于质谱的蛋白质组学服务; 2）提供创新的蛋白质组学数据 分析和生物信息学平台;以及，3）为研究人员提供实验设计咨询 并对用户进行共享资源内几台自主MS仪器的操作培训。来 在上一个赠款期间，PSR的设备进行了大量升级。在赠款期的早期， 在联邦政府资助的奖项的帮助下获得了三台质谱仪：一台Bruker Maxis Q-TOF，一台 Bruker UltrafleXtreme MALDI TOF-TOF和具有电子转移解离的Bruker Amazon离子阱 （ETD）。今年，三个额外的质谱仪，两个高端仪器（一个热轨道阱融合 和Bruker 15 T FTICR沿着Thermo Quantiva三重四极杆用于靶向（MRM）分析）， 安装在PSR中，由两个NIH S10奖项以及OSUCCC和其他机构提供资金 支持.这些最先进的MS仪器将通过以下方式改善为OSUCCC成员提供的服务 提供显著更高通量的鸟枪法蛋白质组学，改进的翻译后修饰分析， 改进代谢组学的同位素精细结构分析，提高能力和数据质量。PSR已 支持了来自所有五个OSUCCC研究项目的37名OSUCCC成员，包括1名K24，1名N 01，8名P01，2名 P50、19个R 01、8个R21、2个RC 2、1个T32和2个U 01。方案支助改革还为74个以上的OSUCCC作出了贡献。 在上一个赠款期间，成员出版物，其中11种出版物具有期刊影响因子 >10. PSR的未来计划包括不断努力开发和采用新的创新技术 和方法进行蛋白质分析，并获得最先进的质谱和色谱 仪器仪表具体而言，PSR将完全整合最近购买的3种主要仪器， 介绍二维和三维层析方法，并提供蛋白质组学数据 一体化PSR利用广泛的机构支持，仅寻求CCSG的14.2%支持 资金Proteomics Shared Resource是Analytics的一部分。

CORE-013: PROTEOMICS SHARED RESOURCE (PSR) PROJECT SUMMARY / ABSTRACT The OSUCCC Proteomics Shared Resource (PSR) provides CCSG investigators access to advanced mass spectrometry (MS) instrumentation, ancillary instrumentation for sample preparation, and expert staff to enable proteomic research, including data analysis. Two tenured faculty experts in mass spectrometry and proteomics, Drs. Michael Freitas (MBCG) and Vicki Wysocki, serve as PSR Director and Senior Faculty Advisor, respectively. They provide scientific leadership to the PSR with expertise in cancer proteomics, protein chemistry, bioinformatics, and mass spectroscopy methods development. The PSR offers numerous proteomics services to users that fall under the categories of protein identification, characterization, and quantification. The ability to confidently identify proteins is the central role of the PSR. Examples of protein characterization analysis include: identification of post-translational modifications, alternate splice forms, de novo protein sequencing and protein-protein interaction analysis. The Specific Aims of the PSR are to: 1) provide advanced mass spectrometry based proteomics services; 2) provide innovative proteomic data analytics and bioinformatics platforms; and, 3) provide consultations with investigators on experiment design and train users on the operation of several self-operated MS instruments within the shared resource. Over the last grant period, there have been substantial upgrades to equipment in the PSR. Early in the grant period, three mass spectrometers were acquired with the help of federally funded awards: a Bruker Maxis Q-TOF, a Bruker UltrafleXtreme MALDI TOF-TOF, and a Bruker AmaZon ion trap with electron transfer dissociation (ETD). This year, three additional mass spectrometers, two high-end instruments (a Thermo Orbitrap Fusion and a Bruker 15 T FTICR along with a Thermo Quantiva triple quadrupole for targeted (MRM) analyses) are to be installed in the PSR with funding from two NIH S10 awards and also OSUCCC and other institutional support. These state-of-the-art MS instruments will improve services offered to OSUCCC members by providing significantly higher throughput shotgun proteomics, improved post-translational modification analysis, improved isotopic fine structure analysis for metabolomics, and greater capacity and data quality. The PSR has supported 37 OSUCCC members from all five OSUCCC research programs including 1 K24, 1 N01, 8 P01s, 2 P50s, 19 R01s, 8 R21s, 2 RC2s, 1 T32, and 2 U01s. The PSR has also contributed to over 74 OSUCCC member publications during the last grant period, 11 of which were in publications with a journal impact factor >10. The future plans for the PSR involve a constant effort to develop and adopt new innovative techniques and methods for protein analysis, and to acquire state-of-the-art mass spectrometry and chromatography instrumentation. Specifically, the PSR will fully integrate the 3 recently purchased major instruments, to introduce methods for 2-dimensional and 3-dimensional chromatography, and provide proteogenomics data integration. The PSR leverages extensive institutional support, and seeks only 14.2% support from CCSG funds. The Proteomics Shared Resource is part of the Analytics Grouping.