Neuroscience Microscopy Core

项目摘要（NMS） 作为三个斯坦福大学神经科学核心之一，神经科学显微镜服务（NMS）提供资本密集型显微镜设备，这往往超出了个别实验室的预算，并对可用的显微镜和图像分析软件进行专家培训，使研究人员能够实现最高质量的结果。荧光显微镜是当前神经科学研究中的一项基本技术，随着遗传编码荧光报告基因和新显微镜技术的不断发展，在可预见的未来仍将如此。在过去的四年中，NMS每年为50至70名PIS提供服务，平均每年使用显微镜和培训时间为3500小时。大部分支持都流向了NIH资助的神经科学实验室。在过去的四年里，NMS一直保持其显微镜服务的更新，增加了一个新的SIM/STORM超分辨率显微镜，一个新的激光扫描共聚焦显微镜，增加了电生理记录能力，其双光子组织切片钻机，并增加了一个新的高性能图像分析工作站配备Neurolucida神经元追踪软件。根据拟议的赠款，NMS将继续提供最先进和最先进的服务，包括努力扩大其超分辨率显微镜和双光子显微镜的使用。

Project Summary (NMS) As one of three Stanford Neuroscience Cores, the Neuroscience Microscopy Service (NMS) provides capital-intensive microscopy equipment which is often beyond the budgets of individual labs, and expert training on the available microscopes and image analysis software to enable researchers to achieve the highest quality results. Fluorescence microscopy is an essential technique in current neuroscience research and, with the continual development of genetically encoded fluorescent reporters and new microscope technologies, will remain so for the foreseeable future. During each of the previous four years, NMS has served between 50 and 70 Pis with an annual average of 3500 hours of microscope usage and training time. The majority of this support has gone to NIH-funded neuroscience labs. Over the last four years, NMS has kept its microscopy services updated with the addition of a new SIM/STORM super-resolution microscope, a new laser scanning confocal microscope, the addition of electrophysiological recording capability to its two­ photon tissue slice rig, and the addition of a new high-powered image analysis workstation equipped with Neurolucida neuron tracing software. Under the proposed grant, NMS will continue to provide the most up-to­-date and cutting edge services, including efforts to expand the use of its super-resolution microscope and two photon microscopes.