MICROSCOPY CORE

网管核心： 1.计算机控制的电动立式荧光显微镜(Zeiss AxioImager)，具有压电式工作台和微扫描CCD摄像机。该显微镜具有良好的阵列层析成像数据采集设备。 2.具有光谱成像能力的倒置激光扫描共聚焦显微镜(Zeiss LSM510Meta)和用于活细胞延时成像的环境控制室。 3.两个直立的双光子显微镜放在一个大的光学台上，共用一对钛宝石飞秒脉冲激光器。这两种显微镜都配备了同时进行双光子成像和双光子光激活的设备。可配置的台式光学器件还允许在双波长下同时成像。A.一台显微镜是为活体成像设计的(Ultima IV，Prairie Technologies，米德尔顿，西雅图)。 B.另一台显微镜用于组织切片成像(Ultima XY，Prairie Technology

NMS Core: 1. A computer-controlled, motorized upright fluorescence microscope (Zeiss Axioimager), with piezo-controlled stage and microscanning CCD camera. This microscope is well-equipped for Array Tomography data acquisition. 2. An inverted, laser scanning confocal microscope with spectral imaging capability (Zeiss LSM510 Meta) and an environmental control chamber for time-lapse imaging of live cells. 3. Two upright two-photon microscopes on a single large optical table, sharing a pair of Ti:sapphire femtosecond pulsed lasers. Both microscopes are equipped for simultaneous two-photon imaging and twophoton photoactivation. Configurable table optics also allow for simultaenous imaging at dual wavelengths. a. One microscope is designed for in vivo imaging (Ultima IV, Prairie Technologies, Middleton, Wl). b. The other microscope is designed for tissue slice imaging (Ultima XY, Prairie Technologi