Flow Cytometry

FCSR摘要 Sidney Kimmel癌症中心流式细胞术共享资源（FCSR）提供最先进的 荧光激活的细胞分选和分析能力的研究人员悉尼金梅尔癌症 中心和费城地区。最常用的应用是多色单元格表面 表型分析、细胞内细胞因子和信号分子检测、转染测量、研究 细胞凋亡、细胞周期分析、细胞增殖和Ca 2+动员动力学的真实的时间分析。的 分选机、分析仪和工作站可对广泛的、可靠的和准确的特性进行分析。 各种生物样本。提供的这些服务可以定制以适应各种实验设计 的领域。FCSR提供关于当前协议、分析技术和数据呈现的咨询。这 资源在协助SKCC成员开展各种直接相关的研究项目方面发挥着关键作用 对癌症的生物学和治疗的理解。该设施目前的目标包括：1）提供 用于分析型细胞计数数据采集和细胞计数细胞分选的高质量和经济实惠的服务 2）与SKCC成员协商，确定分析型细胞仪 协助制定适当的协议和技术，并为以下方面提供技术支持： 分析，包括数据分析和解释和/或使用BD FACS DIVA和Flowjo的培训 3）为SKCC成员提供全天候使用自动分析流式细胞术的培训 用于胜任和高效的常规分析的仪器。

FCSR Summary The Sidney Kimmel Cancer Center Flow Cytometry Shared Resource (FCSR) provides state-of-the-art fluorescence activated cell sorting and analysis capabilities to investigators of the Sidney Kimmel Cancer Center and the Philadelphia region. The most commonly used applications are multi-color cell surface phenotyping, detection of intracellular cytokines and signaling molecules, transfection measurements, studies of apoptosis, cell cycle analysis, cell proliferation, and real time analysis of Ca2+ mobilization kinetics. The sorters, analyzers, and workstations allow for affordable, reliable, and accurate characterization of a wide variety of biological samples. These services provided can be tailored to suit experimental designs in a variety of fields. FCSR provides consultation on current protocols, analysis techniques, and data presentation. This resource plays a critical role in assisting SKCC members performing various research projects directly related to the understanding of the biology and treatment of cancer. Current goals of the facility include: 1) Provide high-quality and affordably priced services for analytical cytometry data acquisition and cytometric cell sorting of various types of biological particles; 2) Consult with SKCC members to determine analytical cytometry needs, aid in the development of appropriate protocols and techniques, and provide technical support for analysis including data analysis and interpretation and/or training in using BD FACS DIVA and FlowJo softwares; 3) Provide training to SKCC members for 24/7 use of automated analytical flow cytometry instrumentation for competent and efficient routine analysis.