Retinal Mechanisms

项目摘要/摘要 由珍妮弗·亨特和大卫·威廉姆斯领导的罗切斯特大学的一个高度合作的团队将在体内将双光子激发荧光眼底镜(TPEFO)从猴子移植到人类身上。这项技术有可能提供有关显微视网膜形态的新信息，并作为一种更好的、客观的手段来评估视网膜功能，这在现有的成像技术中是罕见的。红外自发荧光减少的影响，目前唯一值得注意的，TPEFO的结果将被表征。此外，我们将建立视网膜损伤的阈值。在重复的TPEF成像后监测皮质盲人受试者的视网膜健康状况将确定TPEFO是否可以安全地应用于正常人的眼睛。

PROJECT SUMMARY/ABSTRACT A highly collaborative team at the University of Rochester led by Jennifer Hunter and David Williams will translate in vivo two-photon excited fluorescence ophthalmoscopy (TPEFO) from monkey to human. This technology has the potential to provide new information on microscopic retinal morphology and to serve as a superior, objective measure to assess retinal function, a rare capability among existing imaging technologies. Effects of IR autofluorescence reduction, currently the only noticeable, consequence of TPEFO will be characterized. Additionally, we will establish thresholds for retinal damage. Monitoring retinal health in cortically blind human subjects after repeated TPEF imaging will establish whether TPEFO can be applied safely to normal human eyes.