Flow Cytometry

摘要--流式细胞仪共享资源 共享资源负责人：乔迪·奥昌多，博士 Tisch癌症研究所(TCI)流式细胞术共享资源设施(FCSRF)为TCI成员提供 获得最先进的技术和专业知识，以成功开展科学研究 项目。流式细胞术是一项强大的技术，使研究人员能够同时研究 破译复杂细胞和分子的每秒数千个细胞的多参数特性 健康和疾病中的过程。FCSRF为单电池等常规应用提供服务 快速分离活细胞进行更具创新性和复杂性的生物学研究，如RNAseq， 代谢组学和脂类组学分析。FCSRF由机构流式细胞术共享 资源设施和两个TCI卫星站点。FCSRF配备了4个分析仪，2个小鼠细胞分选器，3个 包含在生物安全2级以上区域的细胞分选器，专门用于分离未固定的人和小鼠样本 感染II类病原体的细胞和1个质量细胞仪(CyTOF)。此外，两个卫星站点是 配有4台分析仪。配备了7名流式细胞仪和质量细胞仪专家，100%致力于 作为设施，SRF提供操作、培训和咨询服务，涵盖流式细胞术的各个方面 包括细胞周期分析，从癌症患者中分离单个细胞，以及鉴定30多种不同的 用质量细胞术检测同一细胞上的细胞表面/细胞内抗原。考虑到这一点，我们的使命是 提供有关流式/质量细胞术和细胞分选的必要仪器和知识，以便 Tisch癌症研究所的研究人员通过基础科学改进人类癌症诊断和治疗， 翻译和临床研究。

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE Shared Resource Leader: Jordi Ochando, PhD The Tisch Cancer Institute (TCI) Flow Cytometry Shared Resource Facility (FCSRF) provides TCI members access to state-of-the-art technology and expertise for the successful development of scientific research projects. Flow cytometry is a powerful technology that enables researchers to study simultaneous multiparametric characteristics of thousands of cells per second to decipher complex cellular and molecular processes in health and disease. The FCSRF offers services for routine applications such as single cell isolation to rapidly isolate live cells conduct more innovative and complex biological studies such as RNAseq, metabolomics, and lipidomics assays. The FCSRF is comprised of the institutional Flow Cytometry Shared Resource Facility and two TCI satellite sites. The FCSRF is equipped with 4 analyzers, 2 mouse cell sorters, 3 cell sorters contained in biosafety level 2+ areas dedicated to isolate non-fixed human samples and mouse cells infected with class II pathogens, and 1 mass cytometer (CyTOF). In addition, two satellite sites are equipped with 4 analyzers. Staffed with 7 flow and mass cytometry specialists committed at 100% to this facility, the SRF provides operational, training, and consultation services covering all aspects of flow cytometry including cell cycle analysis, isolation single cells from cancer patients, and identification of over 30 different cell surface/intracellular antigens on the same cell using mass cytometry. With this in mind, our mission is to provide the necessary instrumentation and knowledge regarding flow/mass cytometry and cell sorting so that Tisch Cancer Institute investigators improve human cancer diagnosis and treatment through basic sciences, translational, and clinical research.