Metabolomics of the Physiologic Heat Stress Response in Latino Construction Workers

项目摘要 环境温度升高对热相关疾病（HRI）的影响越来越受到关注， 特别是对于在炎热环境中艰苦工作的美国人口中的脆弱亚群， 对公众健康构成严重威胁。建筑业中与热有关的平均年死亡率 只有农业工人超过了工人，拉丁美洲建筑工人的工资最高。 在建筑业工作的任何族裔群体的在职发病率和全因死亡率。日常工作在这些 高温和高强度的职业环境没有足够的HRI预防措施可能会挑战 身体的适应能力，并可能导致更快的进展下HRI级联， 在极端情况下会导致多器官衰竭。这项工作的目标是1）获得先进的技能和专业知识 在代谢组学和大数据分析;和2）利用建立的生理生物监测协议，在一个新的 通过增加代谢组学和扩大生物标志物分析，这项工作 将为更大规模的深入研究奠定基础，旨在更全面地了解热量的生理驱动因素 相关疾病及其并发症在代谢水平在这一人群。建立方法， 热应激激活的典型生理途径的候选代谢产物的初步鉴定 反应，与代谢组学特征相关的感兴趣的生物标志物将是至关重要的， 考虑到与热相关的发病率和死亡率的生理驱动因素，以及最终， 热和其他暴露以及与热有关的慢性疾病恶化。未来更大规模的研究基于 这项基础性工作将描述一个人对热的生理反应的代谢途径， 更广泛的职业样本，这将允许记录未来高温的生理影响- 针对弱势工人群体的适应性干预措施。

Project Summary The effects of rising environmental temperatures on heat-related illnesses (HRI) are of increasing concern, particularly for vulnerable subgroups of the US population who work strenuously in hot environments, with HRI emerging as a serious threat to public health. The average annual rates of heat-related death in construction workers is surpassed only by those in agricultural workers, and Latino construction workers have the highest on-the job morbidity and all-cause mortality of any ethnic group working in construction. Routine work in these high heat and high exertion occupational settings without adequate HRI prevention measures may challenge the body's capacity to adapt and may lead to more rapid progression down the HRI cascade, ranging from heat cramps to multi-organ failure in extreme cases. The goal of this work is 1) obtain advanced skills and expertise in metabolomics and big data analytics; and 2) utilize established physiologic biomonitoring protocols in a new group of vulnerable workers with the addition of metabolomics and expanded biomarker analysis. This work will set the foundation for a larger in-depth study aimed to more fully understand the physiologic drivers of heat related illness and its complications at the metabolic level in this population. Establishing the methodology for preliminary identification of candidate metabolites of typical physiologic pathways activated in the heat stress response, and biomarkers of interest that are associated with metabolomic profiles will be paramount for considering the physiologic drivers of heat-related morbidity and mortality, and eventually, interactions between heat and other exposures and heat-related exacerbation of chronic disease. Future, larger studies based on this foundational work will characterize the metabolomic pathways of the physiologic response to heat in a broader occupational sample, which will allow for documentation of the physiologic impacts of future heat- adaptive interventions for vulnerable worker populations.