Ocular Phenotyping Core

眼部表型核心 7.项目/摘要 眼睛结构和功能的非侵入性评估对于眼科的基础和转化研究都是必不可少的。 视觉科学广泛使用的技术包括用于前节和后节生物显微镜检查的裂隙灯， 视网膜电图（ERG，用于可分离成分量的聚集视网膜信号），光学相干断层扫描 (OCT)用于显示后段和前段中的分层组织，通过多种模式对眼底成像 可视化（颜色，自体荧光，基于染料的血管造影，红外反射）和视动 眼球震颤（评估视觉控制、视敏度和对比敏感度）。为了应对日益增长的UAB 视觉研究人员的需要，一个新的“眼表型核心”建立，包括一个全面的 一套仪器，并提供必要的支持，一个合格的博士工作人员，以协助 准确的眼部表型。具体仪器包括Bioptigen 840 nm SD-OCT和Micron IV数字 用于小型动物的眼底照相机、用于大型动物和人类供体眼睛的Spectralis SDOCT以及Optomotry 小动物包括小鼠和斑马鱼的视动性眼球震颤。该核心将支持15 UAB Vision 科学家，包括13名计划中度到广泛使用的科学家和9名目前由NEI R 01资助的科学家。审查 的出版物在两年前的这一申请显示出出色的生产力，使用这些 合作购买的工具，证明这些活动作为一个核心正式化。导演和 该拟议核心的副主任在视网膜电图和OCT方面拥有丰富的出版经验 验证/解释。核心的新方向包括建立MRI成像， 啮齿动物眼中的钙流。此外，还将举办“眼表型分析博览会”， 通过对非眼部科学家产生的小鼠模型进行全眼部表型筛选， 最初是为了回答与他们感兴趣的器官系统有关的问题而产生的。

Ocular Phenotyping Core 7. Project/Summary Abstract Non-invasive assessment of eye structure and function is essential to both basic and translational research in vision science. Widely used technologies include slit lamp for biomicroscopy of anterior and posterior segment, electroretinography (ERG, for massed retinal signal separable into components), optical coherence tomography (OCT) for display of layered tissues in posterior and anterior segments, imaging of the fundus via multiple modes of visualization (color, autofluorescence, dye-based angiography, infrared reflectance), and optokinetic nystagmus (to assess visuomotor control, visual acuity and contrast sensitivity). In response to growing UAB vision researcher needs, a new “Ocular Phenotyping Core” was established to encompass a comprehensive suite of instrumentation and to provide the necessary support for a qualified PhD staff member to assist in accurate ocular phenotyping. Specific instruments include Bioptigen 840 nm SD-OCT and Micron IV digital fundus camera for small animals, Spectralis SDOCT for large animals and human donor eyes, and Optomotry optokinetic nystagmus in small animals including mice and zebrafish. This core will support 15 UAB Vision Scientists, including 13 with planned moderate to extensive use and 9 who are currently NEI R01-funded. Review of publications during the two years prior to this application reveals excellent productivity using these collaboratively purchased instruments, justifying the formalizing of these activities as a core. The Director and Associate Director of this proposed core have extensive publication experience in electroretinography and OCT validation/ interpretation, respectively. New directions for the core include the establishment of MRI imaging for calcium flux in rodent eyes. Additionally an “Ocular Phenotyping Fair” will be implemented to identify new ocular mouse models through full ocular phenotyping screens of mouse models generated by non-ocular scientists that were originally generated to answer questions pertinent to thier organ systems of interest.