Surface antimicrobial coating to prevent bacterial biofilms

项目摘要 植入式医疗器械被广泛用作患者的基本支持。预测水产 许多人在其一生中将使用至少一个这样的设备。不过，还有医疗问题 比如细菌感染。特别是，植入物中细菌生物膜的形成 医疗设备进一步使问题复杂化。生物膜是细菌群落，它们共同作用， 逃避宿主或抗生素的杀伤目前，在医学上还没有针对这种生物膜的治疗方法。 最后一个解决方案是更换受感染的设备。这样的替换是昂贵和痛苦的， 然而.因此，本提案的目的是开发新的抗微生物肽， 防止细菌在医用高分子材料上形成生物膜的固定化技术 意义与传统的抗生素不同，抗菌肽在数百万年后仍然有效。 进化我们推测，不同活性的短抗菌肽的固定化可以 防止在聚对苯二甲酸乙二醇酯（PET）表面上形成生物膜。为了验证我们的假设，我们 具体目的如下：（1）通过序列测定优化抗菌短肽 置换;和（2）将优化的肽附着到聚对苯二甲酸乙二醇酯表面。我们 他们已经做好了充分的准备来完成这个项目。我们已经获得了初步的结果，支持的可行性 在PI的实验室里进行这项研究。该R 03项目的预期结果有两个方面。第一、 这个项目将导致一个具有不同活性的短肽家族对抗超级细菌。二是 新的肽固定化技术的发展将为那些 需要具有改进性能的新的植入医疗装置。最后，该项目还提供了一个 一个极好的培训机会，PI的博士后研究员谁将追求一个独立的职业生涯在这方面 方向

Project Summary Implant medical devices are widely utilized as an essential support to patients. It is projected that many people will use at least one such device during their life time. However, there are medical issues with these devices such as bacterial infection. In particular, the formation of bacterial biofilms in implant medical devices further complicates the problem. Biofilms are bacterial communities that work together to escape the killing by the host or antibiotics. Currently, there is no treatment for such biofilms on medical devices and the last solution is to replace the infected ones. Such a replacement is costly and painful, however. Therefore, the objective of this proposal is to develop novel antimicrobial peptides and immobilization technologies that prevent bacterial biofilm formation on polymer materials of medical significance. Unlike traditional antibiotics, antimicrobial peptides remain potent after millions of years' evolution. We hypothesize that the immobilization of short antimicrobial peptides of varying activities can prevent biofilm formation on the polyethylene terephthalate (PET) surface. To test our hypothesis, we have designed the following specific aims: (1) To optimize the short antimicrobial peptide by sequence permutation; and (2) To immobilize the optimized peptide to the polyethylene terephthalate surface. We are well prepared to pursue this project. We have obtained preliminary results that support the feasibility of this research in the PI's laboratory. The anticipated outcomes of this R03 project are two fold. First, this project will lead to a family of short peptides with varying activity against superbugs. Second, the development of novel peptide immobilization technology will bring forth new possibilities to patients who are in need of new implant medical devices with improved properties. Finally, the project also provides an excellent training opportunity to the PI's postdoctoral fellow who will pursue an independent career in this direction.