ShEEP Request for Cytek Aurora Spectral Flow Cytometer

通过此应用程序，我们从ORD共享设备评估计划(SHEEP)申请资金 购买最先进的多色流式细胞仪，以支持、增强和满足 爱荷华州爱荷华市一群由退伍军人管理局资助的研究人员的专业技术需求。具体来说， 我们寻求获得Cytek Aurora频谱流式细胞仪，它将安装在退伍军人管理局租赁的空间内，具有 方便访问整个用户组。我们的研究组合是多样化的，包括传染病的研究 疾病(利什曼原虫、弗朗西斯氏菌、幽门螺杆菌)、自身免疫性疾病(包括神经炎性疾病和 炎症性肠病、移植物抗宿主病、肥胖性高血压的神经机制 和白血病干细胞的基础研究以及B细胞淋巴瘤的发生。所有这些地区都有 对退伍军人的健康产生重大影响。重要的是，所有这些研究途径都严重依赖于 多参数流式细胞术用于评价机械生物学的各个方面。这需要 拟议的文书能够获得并加强对#年所述问题的科学洞察力 正在进行的项目。随着我们对这些疾病情况的了解越来越多，它变得越来越清楚 细胞群不是同质的，需要作为不同的亚群进行研究，以便 了解它们的确切作用或在治疗环境中调节它们的功能。几种功能分析 现在可以通过流式细胞仪技术进行，与传统方法相比具有明显的优势。 这些应用正在改变我们对不同类型免疫细胞之间相互作用的理解 系统。此外，还可以通过多参数数据的强大利用和分析 生物信息学。收购5激光、24+色Cytek Aurora流式细胞仪将提供尖端技术 这将极大地提高整个用户群体的研究水平。我们在这里介绍 我们建议的理由和理由，列举了文书将如何提供 更深入地了解正在进行的退伍军人管理局资助的研究，并概述我们的坚定承诺，使 这一计划的实施。因此，收购Cytek Aurora将有助于确保我们继续 我们各自领域的领导者站在研究的前沿，以改善退伍军人的健康。

With this application we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a new state-of-the-art multichromatic flow cytometer in order to support, enhance and fulfill the specialized technologic needs of a group of multiple VA-funded researchers at the Iowa City VA. Specifically, we seek to obtain the Cytek Aurora Spectral Flow Cytometer, which will be housed in VA-leased space, with convenient access to the entire user group. Our research portfolio is diverse and includes studies of infectious diseases (Leishmania, Francisella, H. pylori), autoimmune diseases (including neuroinflammatory disease and inflammatory bowel disease), graft-vs-host disease, neuronal mechanisms of obesity-induced hypertension and fundamental study of leukemic stem cells as well as genesis of B-cell lymphoma. All these areas have major impact for the health of veterans. Importantly, all these avenues of research critically rely on multiparameter flow cytometry for evaluation of various aspects of mechanistic biology. This requires the proposed instrument to be able to gain and enhance scientific insight into the questions being addressed in ongoing projects. As our understanding of these disease situations grows, it is becoming increasingly clear that populations of cells are not homogeneous and need to be studied as distinct subpopulations in order to understand their precise role or to modulate their function in a therapeutic setting. Several functional assays can now be performed by flow cytometric technology with a distinct advantage over traditional methodologies. These applications are changing our understanding of the interplay between various cell types of the immune system. In addition, powerful utilization and analysis of multiparametric data can be attained through bioinformatics. The acquisition of the 5-laser, 24+-color Cytek Aurora flow cytometer will provide cutting-edge instrumentation that will dramatically enhance research across the user group. We present here the justification and rationale for our proposal, cite various specific examples of how the instrument would provide greater insight into ongoing VA-funded research and outline our strong commitment that will enable the smooth implementation of this program. Thus, the acquisition of the Cytek Aurora will help ensure that we remain leaders in our respective fields at the forefront of research to improve Veteran’s health.