Meso Scale Discovery SECTOR Imager 6000

描述(由申请人提供)：该提案的目标是购买最先进的多重检测系统，用于检测可溶性分子(细胞因子、生长因子、代谢标志物、激素、炎症介质和其他临床标志物)，以支持、增强和满足德克萨斯大学西南分校一群由NIH资助的研究人员的专业技术需求。主要使用者群体在免疫学、激素生产、细胞信号和分化研究以及可溶性因子生产的不同领域进行研究。所有调查人员都需要拟议的文书，以加强对正在进行的项目中涉及的问题的科学洞察力。随着我们对各种疾病的免疫学基础的了解不断加深，越来越清楚的是，免疫细胞群体并不是同质的，它们以一系列复杂的组合产生细胞因子。这些细胞因子需要以日益多样化和深入的方式进行研究，以便了解它们在细胞间功能调节以及治疗环境中的确切作用。在对生长因子和激素受体的研究中，最近的一些报道表明，这些受体可以作为传感器，协调调节控制其配体的细胞内水平的基因的表达，并诱导细胞分化。与细胞因子一样，单个样本(体液、细胞培养上清液、细胞裂解液)中存在的分子阵列可能非常复杂。虽然这些分子可以通过传统的ELISA法和最近的细胞学珠粒阵列系统进行检测，但在单个样本中同时检测多个分子是不可能的，特别是在可能有少量可用于评估的情况下，就像临床/转化性研究中经常出现的情况一样。在这个新系统中独特的电化学发光检测方法的可用性将使这种多重分析成为可能，并大大增加我们对各种细胞类型与一系列可溶性分子及其受体之间的相互作用的了解。此外，通过生物信息学可以实现对多参数数据的强大利用和分析。我们在这里介绍了我们建议的理由和理由，列举了各种具体的例子，说明该工具将如何为正在进行的NIH资助的研究提供更多的洞察力，并概述将使NCRR SIG计划顺利实施的强有力的机构承诺。 项目简介：对可溶性分子的分析是当代基础、翻译和临床研究的重要组成部分。然而，由于方法不足，解剖复杂生物体液的能力受到阻碍。我们建议购买Meso Scale Discovery Sector(R)Imager 6000读卡器。该仪器具有多路复用、宽动态范围、灵敏度和高通量能力，这些都是德州大学西南分校目前可用的仪器或市场上其他仪器所不具备的。该仪器的独特之处在于它能够同时分析各种样品中存在的多种可溶分子

DESCRIPTION (provided by applicant): The objectives of this proposal are to purchase a state-of-the-art multiplex assay system for the detection of soluble molecules (cytokines, growth factors, metabolic markers, hormones, mediators of inflammation, other clinical markers) in order to support, enhance and fulfill the specialized technologic needs of a group of multiple NIH-funded researchers at UT Southwestern. The major user group conducts research in varied areas of immunology, hormone production, studies of cell signaling and differentiation, and soluble factor production. All investigators require the proposed instrument in order to enhance scientific insight into the questions being addressed in ongoing projects. As our understanding of the immunologic basis of various disease situations grows, it is becoming increasingly clear that populations of immune cells are not homogeneous and produce cytokines in a complex array of combinations. These cytokines need to be studied in an increasingly multiplexed and in depth fashion in order to understand their precise role in intercellular modulation of function as well as in therapeutic settings. In the study of growth factor and hormone receptors, several recent reports indicate that these receptors serve as sensors that can coordinately regulate the expression of genes controlling the intracellular levels of their ligands and induce cell differentiation. As with cytokines, the array of molecules present in a single sample (body fluid, cell culture supernatant, cell lysate) can be extraordinarily complex. While these molecules can be detected by conventional ELISA methods and, more recently, by cytometric bead array systems, robust simultaneous detection of multiple molecules in a single sample has not been possible, especially where small quantities may be available for evaluation, as is often the case in clinical/translational research. Availability of the unique electrochemiluminescence detection methodology in this novel system will allow such multiplexed analyses and add substantially to our understanding of the interplay between various cell types and a host of soluble molecules and their receptors. In addition, powerful utilization and analysis of multi-parametric data can be attained through bioinformatics. We present here the justification and rationale for our proposal, cite various specific examples of how the instrument would provide greater insight into ongoing NIH-funded research and outline the strong institutional commitment that will enable the smooth implementation of the NCRR SIG program. PROJECT NARRATIVE: Analysis of soluble molecules is a critical component of contemporary basic, translational, and clinical research. However, the ability to dissect complex biological fluids is hampered by inadequate methods. We propose to purchase the Meso Scale Discovery SECTOR(r)Imager 6000 reader. The instrument has multiplexing, wide dynamic range, sensitivity, and high throughput capabilities that are not present on currently available instrumentation at UT Southwestern or on other instruments in the market. The instrument is unique in its ability to simultaneously analyze multiple soluble molecules present in a wide variety of samples