Integrated Platform for Mass-Spectrometric Studies of Protein Structure

总结 研究生物分子的高级结构（HOS）和相互作用的技术是 重点介绍了NIGMS SBIR/STTR研究课题。研究的质谱方法 HOS包括羟基自由基足迹以及化学方法共价标记 氨基酸侧链官能团，以及氢/氘交换（HDX）。 蛋白质骨架酰胺。这些方法只需要少量的不一定纯的 材料，可以研究蛋白质在溶液中的构象和动力学， 配体。质谱法特别适合生物制药的可比性 和表位作图研究。目前，还没有软件可以分析质量 来自共价标记和HDX实验的光谱数据，即使这两个 方法既探测溶剂的可及性，如果结合起来，将提供更好的分辨率， 更大的信心其结果将是供应商中立的商业软件， 分析和报告功能，可同时处理HDX和共价标记 实验

Summary Technology for studying the higher order structure (HOS) and interactions of biomolecules is a highlighted NIGMS SBIR/STTR research topic. Mass-spectrometric methods for studying HOS include hydroxyl radical footprinting as well as chemical methods to covalently label amino acid side-chain functional groups, and also hydrogen/deuterium exchange (HDX) on protein backbone amides. These methods require only small amounts of not-necessarily-pure material, and can study conformation and dynamics of proteins in solution with or without ligands. Mass spectrometric methods are especially well suited to biopharma comparability and epitope mapping studies. Currently, there is no software that can analyze mass spectrometric data from both covalent labeling and HDX experiments, even though the two methods both probe solvent accessibility and, if combined, would give finer resolution and greater confidence. The outcome will be vendor-neutral commercial software with advanced analytical and reporting capabilities that can handle both HDX and covalent labeling experiments.