Gene Targeting & Transgenic Shared Resource

基因靶向和转基因共享资源（GeTT）的使命是确保罗斯威尔公园 CCSG成员可以获得最先进的转基因小鼠技术、方法和动物模型。 随着现代哺乳动物基因组操作技术的发展， 小鼠已经成为新的癌症模型的来源，这些模型有助于阐明导致肿瘤的途径。 发展和进步。GeTT已经产生了超过1,000种新的基因工程小鼠品系， CCSG计划成员。最近引入的CRISPR/Cas9基因组编辑技术 大大提高了生成新小鼠模型的效率，并使一些关键的新研究成为可能。 罗斯威尔公园的调查员进行了调查在报告所述期间（2013- 2017年），GeTT共为47个Roswell用户提供服务，其中42个（89%）是CCSG成员。一般来说，GeTT 向其CCSG成员收取的费用约为商业公司转基因产品收费的三分之一。 基因改造小鼠资源主任和共同主任从最早期起就向调查人员提供指导 当构造设计到项目的后期阶段时， 表型分析资源技术人员执行专门的ES细胞和胚胎操作方法 来制造基因工程小鼠。需要开发转基因小鼠模型的项目 正变得越来越相关，以验证来自体外研究和高通量临床研究的发现， 基于数据集。GeTT的具体目标是：1）为了确保罗斯威尔公园CCSG成员， 获得最先进的转基因方法，技术和小鼠模型; 2）作为“一站式” 与遗传工程有关的咨询、教育、培训和专家支持的共享资源 小鼠模型; 3）与罗斯韦尔公园的其他共享资源协同工作，以提供优化的 为CCSG成员提供研究环境。GeTT在提供所有必要的 技术和服务，以实现目标。GeTT未来的计划是增加 通过优化几种新的基于CRISPR的基因组编辑， 技术. GeTT目前正在开发新的技术，如CRISPR和GONAD（Gonadal 核酸递送的输卵管转移）。此外，GeTT最近开发了一项服务， CCSG成员实验室的小鼠群体管理，这些实验室之前没有复杂小鼠的经验 用于队列分析的育种。

The mission of the Gene Targeting and Transgenic Shared Resource (GeTT) is to ensure that Roswell Park CCSG members have access to state-of-the-art transgenic mouse technologies, methods and animal models. With the development of contemporary mammalian genome manipulation technologies, genetically engineered mice have become a source of new cancer models that have helped elucidate pathways contributing to tumor development and progression. GeTT has generated over 1,000 novel genetically engineered mouse lines for CCSG program members. The recent introduction of CRISPR/Cas9 genome editing technologies has drastically improved the efficiency of generating new mouse models, and enabled some critical new research by Roswell Park investigators that would not have been otherwise possible. In the reporting period (2013- 2017), the GeTT served a total of 47 Roswell users, of which 42 (89%) were CCSG members. Typically, GeTT charges its CCSG members ~one-third of what commercial companies would charge to create genetically engineered mice. The Resource Director and Co-Director provide guidance to investigators from the earliest planning stages of the project when constructs are designed to advanced stages of the project during phenotype analysis. Resource technicians perform the specialized ES cell and embryo manipulation methods to generate the genetically engineered mice. Projects requiring the development of transgenic mouse models are becoming increasingly relevant to validate findings from in vitro studies and from high-throughput clinical- based datasets. The Specific Aims of the GeTT are: 1) To ensure that Roswell Park CCSG members, have access to state-of-the-art transgenic methods, technologies, and mouse models; 2) To serve as a “one-stop” Shared Resource for consultation, education, training and expert support related to genetic engineering of mouse models; 3) To work synergistically with other shared resources at Roswell Park to provide the optimized research environment for CCSG members. The GeTT has significant expertise in providing all the necessary technologies and services to achieve the objectives of the Aims. The GeTT future plan is to increase the number of CCSG members utilizing the GeTT by optimizing several new CRISPR-based genome-editing techniques. GeTT is currently developing new techniques such as Easi-CRISPR and GONAD (Gonadal oviductal transfer of Nucleic Acid Delivery). In addition GeTT has recently developed a service that offers mouse colony management to CCSG member labs who do not have previous experience in complex mouse breeding for cohort analysis.