Protein Production and Biochemical Characterization Core

蛋白质核心摘要 高质量的蛋白质对于结构研究的成功至关重要。蛋白质核心将支持任何PCHPI- 通过生产毫克量的同质重组病毒发起项目和合作者 蛋白质、人类细胞蛋白质及其复合体，用于结构研究。核心还将开展 支持和验证蛋白质和蛋白质复合体的生物物理和生物分析特性 结构研究。为此，核心将以迭代的方式与核磁共振核心合作，以产生蛋白质 适用于结构研究，将为X射线核心和冷冻-EM核心提供蛋白质和络合物 筛选和结构测定。核心还将定期与互动进行沟通 发现核心和艾滋病毒病毒学核心，这两个核心一起将向蛋白质核心提供信息 发现新的靶蛋白和/或验证它们，从而使新的复合体准备进入结构 测定管道。最后，核心将通过为项目目标制造蛋白质来支持所有项目。

Protein Core Abstract High quality proteins are essential for the success of structural studies. The Protein Core will support any PCHPI- initiated projects and collaborators by producing milligram quantities of homogeneous recombinant viral protein, human cellular proteins and their complexes for structural studies. The Core will also carry out biophysical and bioanalytical characterization of proteins and protein complexes to support and validate structural studies. To that end the Core will work in an iterative fashion with the NMR Core to generate proteins suitable for structural studies and will provide protein and complexes to the X-ray Core and Cryo-EM Core for screening and structure determination. The Core will also communicate on a regular basis with the Interactions Discovery Core and HIV Virology Core, which together will provide information to the Protein Core on the discovery of novel target proteins and/or their validation, thus getting new complexes ready to enter the structure determination pipeline. Finally, the Core will support all Projects by making proteins for project goals.