Genes Underlying Reproductive Behavior and Physiology

项目摘要/摘要 神经科学研究中的主要挑战之一是在大脑水平上了解大脑的功能 基因、基因调控网络、神经元和电路结构。这项建议旨在了解果蝇 生殖行为，一个高度易处理的模型，给出了主要调控转录因子的知识 以及支撑这些行为的神经解剖结构。无果(FRU)编码一组主法规 转录因子，这是必要的，并在很大程度上足以确定先天男性的潜力 求爱行为。这一建议是建立在我们的结果基础上的，该结果证明了一系列细胞黏附 分子，称为dprs和dips，在确定生殖行为的潜力方面具有重要功能。 FRU的下游。我们将确定重叠的FRU表达神经元子集的行为所起的作用 DPR/DIP表达(FU P1∩DPR/DIP)，采用遗传交叉策略。我们还将确定如何 FRU P1神经元的解剖性二型性是通过研究DPR/DIPS的作用而产生的。这 研究还将阐明Fru表达神经元中的DPR/DIP相互作用组。使用特定于单元格类型 利用基因组学工具，我们还将对FU P1∩DPR/DIP表达亚群进行分子分类，以阐明 产生不同神经功能的分子基础。

Project Summary/Abstract One of the major challenges in neuroscience research is to understand the function of the brain at the level of genes, gene regulatory networks, neurons, and circuit structure. This proposal seeks to understand Drosophila reproductive behaviors, a highly tractable model, given knowledge of the master regulatory transcription factors and neuroanatomical structures that underlie these behaviors. fruitless (fru) encodes a set of master regulatory transcription factors, which are necessary and largely sufficient for specifying the potential for innate male courtship behaviors. This proposal is built on our results that demonstrate that a family of cell adhesion molecules, called Dprs and DIPs, have important functions in specifying the potential for reproductive behavior downstream of fru. We will determine the roles for behavior of subsets of fru-expressing neurons that overlap with Dpr/DIP expression (fru P1∩Dpr/Dip), using genetic intersectional strategies. We will also determine how anatomical sexual dimorphism in fru P1 neurons arises by functionally examining the roles of Dpr/DIPs. This study will also elucidate the Dpr/DIP interactome within fru-expressing neurons. Using cell-type specific genomic tools, we will also molecularly classify fru P1∩Dpr/Dip-expressing subpopulations to elucidate the molecular underpinnings that give rise to different neuronal functions.