Design and Evaluation of a Plasmodium vivax ultra-dense peptide array

疟疾寄生虫间日疟原虫威胁着世界一半的人口。同时进行淘汰赛 虽然世界各地的努力正在成功地减轻恶性疟疾的负担，但情况并不乐观 对间日疟原虫来说。这种差异的部分原因是我们对间日疟原虫生物学的了解很差，这是很困难的。 在实验室里学习。因此，对我们免疫系统识别的寄生虫蛋白有更好的了解 可以指导疫苗开发，实现对传播和接触的更严格评估，并允许 识别携带潜伏寄生虫的个体。不幸的是，对P. 间日疟原虫的蛋白质最多只能含有几百种蛋白质，而这些蛋白质通常来自血液阶段。 寄生虫。我们建议在这里利用由我们和其他人生成的大量基因组和转录组数据 以生成在整个生命周期中表达的假定蛋白质编码转录本的全面列表 寄生虫。我们将使用高密度多肽阵列设计和评估所有这些蛋白质的血清反应性。 以及经过广泛鉴定的血清样本。我们的研究将提供一个全面的 透视间日疟原虫在感染过程中被宿主识别并构成坚实基础的蛋白 用于确定候选疫苗和用于测量暴露和传播的生物标志物，以及 潜在的，与肝脏中存在休眠寄生虫相关的标志物。

The malaria parasite Plasmodium vivax threatens half of the world's population. While on-going elimination efforts are successfully reducing the burden of falciparum malaria worldwide, the situation is much less promising for P. vivax. This discrepancy is partially due to our poor understanding of the biology of P. vivax that is difficult to study in the laboratory. Thus, a better understanding of the parasite proteins recognized by our immune system could guide vaccine development, enable more rigorous assessments of transmission and exposure, and allow the identification of individuals carrying dormant parasites. Unfortunately, analyses of the seroreactivity of P. vivax proteins have been limited to, at best, a few hundred proteins, and those typically derive from blood-stage parasites. We propose here to leverage extensive genomic and transcriptomic data generated by us and others to generate a comprehensive list of putative protein-coding transcripts expressed throughout the life cycle of the parasites. We will design and evaluate the seroreactivity of all those proteins using a high-density peptide array and serum samples that have been extensively characterized. Our studies will provide a comprehensive perspective on the P. vivax proteins recognized by the host during an infection, and constitute a solid foundation for identifying vaccine candidates and biomarkers for measuring exposure and transmission, as well as potentially, markers associated with the presence of dormant parasites in the liver.