Expanding xenosurveillance capabilities in central America

这项提议是为了R21，扩大中美洲的异种监视能力。该项目是一项高度 包括病毒学、公共卫生昆虫学专业知识的合作者在内的多学科努力， 监测，以及人类临床和兽医学。我们的研究是基于这样一个观点，即蚊子可以 作为监测工具，因为它们本质上是自主的血液采样设备。刚喂饱的 蚊子是从住所内收集的，血液被用于监测目的。如果成功，我们的 这项工作将产生简化某些类型的需要人类血液的监测的影响，因为 尽管大多数人讨厌自己和孩子被针扎着，但他们似乎并不介意 蚊子被从家里赶走。此外，我们的方法有可能使 从动物身上取样更安全，因为大大减少了与恐惧和愤怒的动物直接接触的需要， 咬、踢等。 目标1侧重于通过与以人类为基础的综合征进行直接比较来验证异种监控 监视系统。目标2旨在通过获得以下各项的定量估计来保持我们工作的科学严谨性 CX的敏感性。Ququiefasciatus和Ae.埃及伊格普提用于异种监视。 这一提议利用了先前在异种监测方面所做的大量工作，这些工作的重点是一个。W.冈比亚亚纲 非洲，以及正在进行的危地马拉农村异种监测试点研究。

This proposal is for an R21, Expanding xenosurveillance capabilities in Central America. The project is a highly multidisciplinary effort that includes collaborators with expertise in virology, public health entomology, surveillance, and human clinical and veterinary medicine. Our study is based on the idea that mosquitoes can serve as surveillance tools because they are essentially autonomous blood sampling devices. Freshly fed mosquitoes are collected from within dwellings and blood is used for surveillance purposes. If successful, our work would have the impact of simplifying certain types of surveillance that require human blood because although most people hate it when they and their children are stuck with needles, they don’t seem to mind mosquitoes being removed from within their homes. In addition, our approach has the potential to make sampling from animals safer by vastly reducing the need for direct contact with fearful and angry animals that bite, kick, etc.. Aim 1 focuses on validating xenosurveillance through a direct comparison to human-based syndromic surveillance. Aim 2 is designed to maintain the scientific rigor of our work by obtaining quantitative estimates of the sensitivity of Cx. quinquefasciatus and Ae. aegypti for xenosurveillance. This proposal leverages significant prior work on xenosurveillance that has focused on An. gambiae in W. Africa, and an ongoing pilot study of xenosurveillance in rural Guatemala.