Whole genome sequencing laboratory, procurement of supplies and reagents and equipment maintenance for surveillance, outbreak assessment, and data mining.

摘要： 路易斯安那州动物疾病诊断实验室(LADDL)是四个全基因组之一 Vet-LIRN选定的参与试点项目的测序实验室 全基因组测序(WGS)监测人畜共患病的耐药性 病原体。这项提案的长期目标是增加来自动物或 国家抗菌素耐药性监测系统中饲料细菌的分离 并开发快速反应网络，以准确解析假定的动物性食品- 传播的疾病暴发。该项目有3个具体目标：1.为 Vet-LIRN继续试验所需的用品、试剂和设备维护 抗菌素耐药性(AMR)和测序计划。2.通过全基因组 测序：a)监测动物饲料和 受感染的动物；b)建立参考数据库，以帮助解决食源性疫情 更有效率；以及c)通过将食物来源和 一连串的疾病。3.为诊断病例或病原体提供支持和检测 CVM-Vet-LIRN提交的利息。 抗菌素耐药性对人类和动物都是一个持续且日益严重的威胁 健康。全基因组测序是最近和更强大的工具之一 研究它，监测它的发展和进展。测序结果也表明 在食源性疾病暴发的流行病学调查中具有极好的实用性。

Summary: Louisiana Animal Disease Diagnostic Laboratory (LADDL) is one of four whole genome sequencing laboratories selected by Vet-LIRN to participate in a pilot project using Whole Genome Sequencing (WGS) to monitor antimicrobial resistance in zoonotic pathogens. Long term goals of this proposal are to add genomic data from animal or animal feed bacterial isolates to National Antimicrobial Resistance Monitoring System and to develop a rapid response network for accurate resolution of putative animal food- borne disease outbreaks. This project has 3 specific aims: 1. Provide funding for supplies, reagents and equipment maintenance for continuation of Vet-LIRN’s pilot Antimicrobial Resistance (AMR) and Sequencing Project. 2. Through whole genome sequencing: a) provide surveillance of antimicrobial resistance in animal feeds and in infected animals; b) build reference databases to aid in solving foodborne outbreaks more efficiently; and c) aid foodborne disease investigations by linking food sources and clusters of disease. 3. Provide support and testing for diagnostic cases or pathogens of interest referred by CVM-Vet-LIRN. Antimicrobial resistance is a constant and growing threat to both human and animal health. Whole genome sequencing is one of the recent and more powerful tools to study it and monitor its development and progression. Sequencing also has shown excellent utility in the epidemiological investigations of food-borne disease outbreaks.