Vet-LIRN Network Capacity-Building Project: Bacterial Whole Genome Sequencing

总结/摘要： 需要增加全基因组测序（WGS）以提供抗生素耐药性 在实验室网络中对新出现的抗生素耐药性病原体进行基因型检测， 目前的健康概念。抗生素耐药性是一个持续和不断增长的威胁， 人类和动物健康。WGS是一个最近和更强大的工具来研究和监测 抗药性病原体出现的发展和进展。此外，WGS 提供用于建立公共基因组数据库的实时序列数据， 食源性细菌病原体的遗传特征，临床标本分离株， 和调查卫生保健设施中的疫情，并提供感染信息 控制措施。对WGS等新技术的持续投资对于 支持不断扩大的检测量，同时维持快速检测和公共卫生 反应测序也被证明是流行病学的一个很好的工具， 调查食源性疾病暴发，以确定抗生素耐药性 仪表板，并供临床医生用于做出最佳治疗决策。路易斯安那 动物疾病诊断实验室（LANDO）作为全基因组测序（WGS） 兽医医学中心（CVM）Vet-LIRN兽医诊断实验室 实验室计划。LASTON是Vet-LIRN最初选择的五个实验室之一， 参与一个试点项目，使用WGS监测人畜共患病和 新兴病原体这项提案的长期目标是将整个基因组序列 来自动物临床标本或动物饲料的细菌分离株的基因组数据 细菌分离株到国家抗菌药物耐药性监测系统，并开发一种快速 应对网络，以准确解决假定的动物食源性疾病暴发。的 该项目有三个具体目标。第一，提供继续开展兽医工作所需的资金， LIRN的抗生素耐药性（AMR）和测序试点项目。主要成本 该项目用于购买用品和试剂以及支付设备维修费 合同.其次，对Vet-LIRN源实验室提交的分离株进行WGS， 基因组信息，用于鉴定抗微生物剂抗性细菌或病原体 监测或疾病爆发。最后，为了向 国家数据库，以帮助在基因组和生物医学信息需要跟踪细菌 CVM和美国食品药品监督管理局（FDA）关注的病原体。

Summary/Abstract: The need to increase Whole-genome sequencing (WGS) to provide antibiotic resistance genotypic testing in laboratory networks for emerging antibiotic resistance pathogens is critical for the current One Health Concept. Antimicrobial resistance is a constant and growing threat to human and animal health. WGS is one of the recent and more powerful tools to study and monitor the development and progression of the emergence of antibiotic-resistant pathogens. Also, WGS provides real-time sequence data used to build public genomic databases that aid in identifying the genetic characterization of foodborne bacterial pathogens, isolates from clinical specimens, and investigation of outbreaks in health care facilities and to provide information for infection control practices. The continued investment in new technologies such as WGS is crucial for supporting an expanding testing volume while sustaining rapid detection and public health response. Sequencing has also been shown to be an excellent utility for epidemiological investigations of foodborne disease outbreaks, for establishing antimicrobial-resistant dashboards, and for use by clinicians for making the best treatment decisions. The Louisiana Animal Disease Diagnostic Laboratory (LADDL) serves as a whole genome sequencing (WGS) laboratory for the Center for Veterinary Medicine (CVM) Vet-LIRN Veterinary Diagnostic Laboratory Program. LADDL is one of five laboratories originally selected by Vet-LIRN to participate in a pilot project using WGS to monitor antimicrobial resistance in zoonotic and emerging pathogens. Long term goals of this proposal are to add whole genomic sequence genomic data from bacterial isolates originating from animal clinical specimens or animal feed bacterial isolates to National Antimicrobial Resistance Monitoring System and to develop a rapid response network for accurate resolution of putative animal foodborne disease outbreaks. The project has three specific aims. First, to provide the funding required for continuation of Vet- LIRN’s pilot Antimicrobial Resistance (AMR) and Sequencing Project. The primary cost of the project is for purchasing supplies and reagents and for payment of equipment maintenance contracts. Second, to perform WGS on isolates submitted by the Vet-LIRN source labs as genomic information for identifying antimicrobial-resistant bacteria or pathogens related to surveillance or disease outbreaks. Finally, to populate and contribute sequence data to the national databases to aid in the genomic and biomedical information needed to track bacterial pathogens of interest to the CVM and U.S. Food and Drug Administration (FDA).