Vet-LIRN Network Capacity-Building Project: Bacterial Whole Genome Sequencing

摘要/摘要： 需要增加全基因组测序(WGS)以提供抗生素耐药性 在实验室网络中对新出现的抗生素耐药病原体进行基因分型测试对于 当前的One Health概念。抗菌素耐药性是一个持续且日益严重的威胁 人类和动物健康。WGS是最新的、更强大的研究和监控工具之一 抗生素耐药病原体出现的发展和进展。另外，WGS 提供用于构建公共基因组数据库的实时序列数据，帮助识别 食源性致病菌、临床标本分离株、 和调查卫生保健设施中的暴发，并提供感染信息 控制实践。对WGS等新技术的持续投资对 支持不断扩大的检测量，同时保持快速检测和公共卫生 回应。测序也被证明是流行病学的一种极好的工具 调查食源性疾病暴发，以建立抗菌素耐药性 仪表板，供临床医生用于做出最佳治疗决策。路易斯安那州 动物疾病诊断实验室(LADDL)作为全基因组测序(WGS) 兽医中心VET-LIRN兽医诊断实验室 实验室计划。LADDL是Vet-LIRN最初选择的五个实验室之一 参与使用WGS监测人畜共患疾病抗菌素耐药性的试点项目 新出现的病原体。这项提议的长期目标是增加整个基因组序列 来源于动物临床标本或动物饲料的细菌分离株的基因组数据 细菌分离株进入国家耐药监测体系并快速发展 准确解决疑似动物食源性疾病暴发的反应网络。这个 该项目有三个具体目标。第一，为继续推行兽医计划提供所需经费： LIRN的试点抗菌素耐药性(AMR)和测序项目。的主要成本 该项目用于采购用品和试剂以及支付设备维护费。 合同。第二，对Vet-LIRN来源实验室提交的分离物进行WGS 用于识别与以下相关的抗菌素耐药细菌或病原体的基因组信息 监测或疾病暴发。最后，要填充序列数据并将其提供给 帮助提供追踪细菌所需的基因组和生物医学信息的国家数据库 CVM和美国食品和药物管理局(FDA)感兴趣的病原体。

Summary/Abstract: The need to increase Whole-genome sequencing (WGS) to provide antibiotic resistance genotypic testing in laboratory networks for emerging antibiotic resistance pathogens is critical for the current One Health Concept. Antimicrobial resistance is a constant and growing threat to human and animal health. WGS is one of the recent and more powerful tools to study and monitor the development and progression of the emergence of antibiotic-resistant pathogens. Also, WGS provides real-time sequence data used to build public genomic databases that aid in identifying the genetic characterization of foodborne bacterial pathogens, isolates from clinical specimens, and investigation of outbreaks in health care facilities and to provide information for infection control practices. The continued investment in new technologies such as WGS is crucial for supporting an expanding testing volume while sustaining rapid detection and public health response. Sequencing has also been shown to be an excellent utility for epidemiological investigations of foodborne disease outbreaks, for establishing antimicrobial-resistant dashboards, and for use by clinicians for making the best treatment decisions. The Louisiana Animal Disease Diagnostic Laboratory (LADDL) serves as a whole genome sequencing (WGS) laboratory for the Center for Veterinary Medicine (CVM) Vet-LIRN Veterinary Diagnostic Laboratory Program. LADDL is one of five laboratories originally selected by Vet-LIRN to participate in a pilot project using WGS to monitor antimicrobial resistance in zoonotic and emerging pathogens. Long term goals of this proposal are to add whole genomic sequence genomic data from bacterial isolates originating from animal clinical specimens or animal feed bacterial isolates to National Antimicrobial Resistance Monitoring System and to develop a rapid response network for accurate resolution of putative animal foodborne disease outbreaks. The project has three specific aims. First, to provide the funding required for continuation of Vet- LIRN’s pilot Antimicrobial Resistance (AMR) and Sequencing Project. The primary cost of the project is for purchasing supplies and reagents and for payment of equipment maintenance contracts. Second, to perform WGS on isolates submitted by the Vet-LIRN source labs as genomic information for identifying antimicrobial-resistant bacteria or pathogens related to surveillance or disease outbreaks. Finally, to populate and contribute sequence data to the national databases to aid in the genomic and biomedical information needed to track bacterial pathogens of interest to the CVM and U.S. Food and Drug Administration (FDA).