Gene Targeting & Transgenic Shared Resource

基因打靶和转基因共享资源(GETT)的使命是确保罗斯韦尔公园 CCSG成员可以获得最先进的转基因小鼠技术、方法和动物模型。 随着当代哺乳动物基因组操作技术的发展，基因工程 小鼠已经成为新的癌症模型的来源，这些模型有助于阐明导致肿瘤的途径 发展和进步。Gett已经为人类培育了1000多个新的基因工程小鼠品系 CCSG计划成员。最近引入的CRISPR/Cas9基因组编辑技术已经 极大地提高了生成新鼠标模型的效率，并使一些关键的新研究成为可能 罗斯韦尔公园的调查人员认为，如果不是这样，这是不可能的。在本报告所述期间(2013年-- 2017年)，Gett总共为47名Roswell用户提供服务，其中42名(89%)是CCSG成员。通常，Gett 向其CCSG成员收取~商业公司创造基因所需费用的三分之一 转基因小鼠。资源总监和联合总监从一开始就为调查人员提供指导 项目的规划阶段当建筑设计到项目的高级阶段时 表型分析。资源技术人员执行专门的胚胎干细胞和胚胎操作方法 以产生基因工程小鼠。需要开发转基因小鼠模型的项目 在验证来自体外研究和高通量临床研究的结果方面正变得越来越重要 基于数据集。GETT的具体目标是：1)确保罗斯威尔公园CCSG成员 获得最先进的转基因方法、技术和小鼠模型；2)提供“一站式”服务 与遗传工程有关的咨询、教育、培训和专家支持共享资源 鼠标模型；3)与罗斯韦尔公园的其他共享资源协同工作，提供优化的 CCSG成员的研究环境。Gett拥有丰富的专业知识，可以提供所有必要的 技术和服务，以实现目标的目标。Gett未来的计划是增加 通过优化几个新的基于CRISPR的基因组编辑来利用GETT的CCSG成员数量 技巧。Gett目前正在开发新的技术，如EASI-CRISPR和性腺(性腺 核酸输送的输卵管转移)。此外，Gett最近开发了一项服务，提供 CCSG成员实验室对没有复杂鼠标经验的人进行鼠群管理 用于队列分析的育种。

The mission of the Gene Targeting and Transgenic Shared Resource (GeTT) is to ensure that Roswell Park CCSG members have access to state-of-the-art transgenic mouse technologies, methods and animal models. With the development of contemporary mammalian genome manipulation technologies, genetically engineered mice have become a source of new cancer models that have helped elucidate pathways contributing to tumor development and progression. GeTT has generated over 1,000 novel genetically engineered mouse lines for CCSG program members. The recent introduction of CRISPR/Cas9 genome editing technologies has drastically improved the efficiency of generating new mouse models, and enabled some critical new research by Roswell Park investigators that would not have been otherwise possible. In the reporting period (2013- 2017), the GeTT served a total of 47 Roswell users, of which 42 (89%) were CCSG members. Typically, GeTT charges its CCSG members ~one-third of what commercial companies would charge to create genetically engineered mice. The Resource Director and Co-Director provide guidance to investigators from the earliest planning stages of the project when constructs are designed to advanced stages of the project during phenotype analysis. Resource technicians perform the specialized ES cell and embryo manipulation methods to generate the genetically engineered mice. Projects requiring the development of transgenic mouse models are becoming increasingly relevant to validate findings from in vitro studies and from high-throughput clinical- based datasets. The Specific Aims of the GeTT are: 1) To ensure that Roswell Park CCSG members, have access to state-of-the-art transgenic methods, technologies, and mouse models; 2) To serve as a “one-stop” Shared Resource for consultation, education, training and expert support related to genetic engineering of mouse models; 3) To work synergistically with other shared resources at Roswell Park to provide the optimized research environment for CCSG members. The GeTT has significant expertise in providing all the necessary technologies and services to achieve the objectives of the Aims. The GeTT future plan is to increase the number of CCSG members utilizing the GeTT by optimizing several new CRISPR-based genome-editing techniques. GeTT is currently developing new techniques such as Easi-CRISPR and GONAD (Gonadal oviductal transfer of Nucleic Acid Delivery). In addition GeTT has recently developed a service that offers mouse colony management to CCSG member labs who do not have previous experience in complex mouse breeding for cohort analysis.