Analytical and Translational Genomics Shared Resource

分析与翻译基因组学共享资源 摘要 联合国儿童基金会分析和翻译基因组学(基因组学)共享资源(前身为联合国儿童基金会Keck- UNM基因组学资源)通过提供具有成本效益的、完整的 服务下一代测序(NGS)和数据分析、微阵列、实时PCR和其他基因组学- 相关技术，包括对患者样本的基因组分析，向联森特派团成员和非成员提供 用户。学院主任Scott Ness博士负责监督该资源，该资源由包括以下人员在内的全职人员操作 经验丰富的湿法实验室研究技术人员外加一支执行初始生物信息学和数据分析的团队。这个 联合国排雷行动中心研究管理处管理该资源。在上一个资助期内，资源 经历了从主要提供基于微阵列的分析到对NGS的新强调的重大转变 服务包括靶向(如基因面板)测序和外显子组测序以及RNA-SEQ和芯片- SEQ使用其离子激流和离子质子测序仪器。该资源维护着一个专用的迷你 用于存储大型数据集的集群，并促进对NGS数据的综合生物信息学分析。这个 下一代测序服务是对现有服务的补充，通过其已建立的 Affymetrix仪器和实时定量聚合酶链式反应检测。资源执行初始处理 利用先进的统计工具和软件，为用户详细分析微阵列和NGS数据 打包并遵守严格的质量控制指南，并与生物统计共享资源进行交互 而生物信息学和高维数据分析共享资源为用户提供了一个完整的 服务范围从详细的实验设计到先进的生物信息学和统计数据 分析。预订、账单和使用情况通过一个集中的在线系统进行跟踪。《资源》 主任和工作人员与联合国排雷行动委员会研究方案密切合作，开发新的方法，以促进 创新的基础和翻译研究目标和资源积极促进和传播 通过维护最新的网站提供有关新的基因组学技术和数据分析技术的信息 佩奇，每月组织专注于翻译基因组学和生物信息学的会议，并通过给予 在联东特派团会议和务虚会上所作的介绍。在上一个五年项目期间，联东特派团33名成员 联发办的3个研究方案使用了资源，共出版了32份出版物，其中4份是 目前悬而未决的PMCID。在报告的2013年7月至2014年6月期间，联东特派团成员负责 占总资源使用量的88%，并得到109项同行审查赠款的支持。

ANALYTICAL & TRANSLATIONAL GENOMICS SHARED RESOURCE ABSTRACT The UNMCC Analytical and Translational Genomics (Genomics) Shared Resource (formerly the UNMCC Keck- UNM Genomics Resource) supports basic and translational cancer research by providing cost-effective, full service next-generation sequencing (NGS) and data analysis, microarray, real-time PCR and other genomics- related technologies, including the genomic analysis of patient samples, to UNMCC members and non-member users. Faculty Director Scott Ness, Ph.D. supervises the Resource, which is operated by a full time staff including experienced wet lab research technicians plus a team performing initial bioinformatics and data analysis. The UNMCC Research Administration manages the Resource. During the previous funding period the Resource underwent a major transition from primarily providing microarray-based assays to a new emphasis on NGS services including targeted (e.g. gene panel) sequencing and exome sequencing as well as RNA-seq and ChIP- seq using its Ion Torrent and Ion Proton sequencing instruments. The Resource maintains a dedicated mini- cluster for storage of large data sets and to promote the integrated bioinformatics analyses of NGS data. The next generation sequencing services complement the existing services provided through its established Affymetrix instruments and with quantitative real-time PCR assays. The Resource performs initial processing and detailed analysis of microarray and NGS data for users, utilizing sophisticated statistical tools and software packages and adhering to strict quality control guidelines, and interacts with the Biostatistics Shared Resource and the Bioinformatics and High Dimensional Data Analysis Shared Resource to provide users with a complete range of services from detailed experimental design through advanced bioinformatics and statistical data analysis. Reservations, billing and usage are tracked through a centralized on-line system. The Resource Director and staff work closely with UNMCC research programs to develop new methodologies that promote innovative basic and translational research goals and the Resource actively facilitates and disseminates information about new genomics technologies and data analysis techniques by maintaining an up-to-date web page, organizing monthly meetings focused on translational genomics and bioinformatics and by giving presentations at UNMCC meetings and retreats. During the previous 5-yr project period, 33 UNMCC members from 3 UNMCC Research Programs used the Resource, resulting in a total of 32 publications, of which 4 are presently pending PMCIDs. In the reporting year of July 2013 – June 2014, UNMCC members were responsible for 88% of total Resource usage and were supported by 109 peer-reviewed grants.