Biospecimen/Bioimaging Core

摘要-生物显微镜/生物成像核心 Biosecimen/生物成像核心利用了斯坦福大学泌尿外科和病理学的基础设施 但增加了服务，使奥布莱恩中心的调查人员受益。在一支合格的队伍带领下， 集中的资源提供了质量优势和规模效率。核心采购并提供 中心调查人员需要生物标记物，包括新鲜的、新鲜冷冻的和福尔马林固定的石蜡- 嵌入性(FFPE)前列腺问题。核心也执行不太显眼但同样重要的活动， 包括患者同意、质量控制/保证、数据库管理、临床病理注释以及 监管合规性。此外，核心还提供组织定性服务，包括组织学、 利用MIBI-TOF进行组织微阵列、激光显微切割和多重免疫组织化学(IHC) (按飞行时间显示的多路复用离子束成像)MIBI-TOF是一项功能强大、功能强大的技术 利用金属标记抗体同时定量40个或更多抗体靶标， 卓越的空间分辨率和动态范围。Core还存储图像并提供对图像的门户访问 包括前列腺核磁共振、整体组织学和IHC，以及MIBI-TOF数据，以及集成的多 从磁共振成像到组织学、细胞和分子的前列腺地图集。生物显微镜/生物成像核心 提供基本的组织采购、定性和成像服务，以支持三个中心 项目、教育丰富和机会池项目，以及奥布莱恩中心以外的项目，第20页 Grant计划，以及更广泛的良性泌尿科社区，。

ABSTRACT – BIOSPECIMEN / BIOIMAGING CORE The Biospecimen/Bioimaging Core leverages infrastructure of the Stanford Urology and Pathology departments, but augments services to benefit O’Brien Center investigators. Led by a qualified team, the centralized resource provides qualitative advantages and efficiencies of scale. The Core procures and provides Center investigators with needed biospecimens, including fresh, freshly-frozen, and formalin-fixed paraffin- embedded (FFPE) prostate issues. The Core also performs less visible but equally important activities, including patient consent, quality control/assurance, database management, clinicopathologic annotation, and regulatory compliance. In addition, the Core provides tissue characterization services, including histology, tissue microarrays, laser microdissection, and multiplex immunohistochemistry (IHC) using MIBI-TOF (Multiplexed Ion Beam Imaging by Time of Flight). MIBI-TOF is a powerful and robust technology that harnesses metal-tagged antibodies for the simultaneous quantification of 40 or more antibody targets, with superior spatial resolution and dynamic range. The Core also stores and provides portal access to images including prostate MRIs, whole-mount histology and IHC, and MIBI-TOF data, as well as an integrated multi- scale prostate Atlas spanning MRI to histology, cells and molecules. The Biospecimen/Bioimaging Core provides essential tissue procurement, characterization and imaging services to support the three Center Projects, the Education Enrichment and Opportunity Pool programs, as well as outside O’Brien Centers, P20 grant programs, and the broader benign urology community, .