Automated Liquid Handler

7.项目总结/摘要 该提案要求为购买美国制造的汉密尔顿NGS星星液体处理平台提供资金， 包括安装、培训和内华达州里诺的汉密尔顿公司提供的12个月保修。机构支助 是为了帮助支付采购成本，并支付设施成本，人员工资和服务合同， 仪器的寿命。该系统将与我们现有的下一代测序系统一起使用 使用包括Illumina NovaSeq 6000、Illumina NextSeq 500，两个Illumina MiSeq平台， 美国国家癌症研究所（NCI）的10 xGenomics Chromium控制器和GenapSys测序平台- 指定Sidney Kimmel癌症中心（SKCC）的MetaOmics共享资源核心设施， 位于费城中部的托马斯杰斐逊大学。Hamliton NGS星星将使下一代 TJU的测序需求，包括SKCC及其区域联盟合作伙伴德雷克塞尔大学。成立 2006年，元组学共享资源通过促进基因组学研究， 数百个研究项目利用各种各样的实验系统。MetaOmics共享资源 最初提供高通量测序，使用两个生命技术/应用生物系统5500 xl遗传 分析仪和两台Ion Torrent PGM仪器，由Illumina系统取代，包括 NovaSeq 6000，提供任何市售测序仪器的最高通量。的 2021年通过S10赠款收购的NovaSeq 6000将从自动化样品处理中受益匪浅 以实现最佳操作。这些平台的可用性刺激了测序需求的增长， 我们的手动处理能力。收购汉密尔顿NGS星星平台将确保实验室 在该设施的支持下，特别是本文所述的NIH资助的研究人员，以及年轻的 研究人员谁依靠元组学共享资源的资源，他们的第一个NIH拨款建议，将继续 以获得及时和具有成本效益的服务。汉密尔顿NGS星星与Illumina协同工作 测序系统提供更高的精度、再现性、更大的样品容量、更快的周转时间和 更具成本效益的测序。提高效率和节约成本将支持和加快以下领域的研究： 遗传性疾病、复杂性状和肿瘤发生、转移和治疗反应的机制。 该提案的优势包括：来自NCI指定的SKCC和TJU管理部门的支持; PI和关键人员的经验和生物技术专业知识; TJU的大规模基础设施 学术，临床和创新支柱;具有创新研究项目的高产教师。采集 这些设备将对TJU的生物医学研究产生长期影响。

7. PROJECT SUMMARY/ABSTRACT This proposal requests funds for purchase of a US-manufactured Hamilton NGS STAR liquid handling platform, including installation, training, and a 12-month warranty from Hamilton Company, Reno, NV. Institutional support is to help defray purchasing cost and to cover facility costs, personnel salaries, and the service contract for the lifetime of the instrument. The system will be housed with our existing next generation sequencing instrumentation including the Illumina NovaSeq 6000, Illumina NextSeq 500, two Illumina MiSeq platforms, 10xGenomics Chromium Controller, and GenapSys sequencing platforms in the National Cancer Institute (NCI)- designated Sidney Kimmel Cancer Center (SKCC) fee-for-service MetaOmics Shared Resource core facility at Thomas Jefferson University (TJU) in central Philadelphia. The Hamliton NGS STAR will enable next-generation sequencing demands at TJU, including the SKCC and its regional consortium partner Drexel University. Founded in 2006, the MetaOmics Shared Resource has been instrumental in promoting genomics research by facilitating hundreds of research projects utilizing a wide variety of experimental systems. The MetaOmics Shared Resource initially offered high-throughput sequencing using two Life Technologies/Applied Biosystems 5500xl Genetic Analyzers and two Ion Torrent PGM instruments, which were replaced by Illumina systems including the NovaSeq 6000, offering the highest throughput of any commercially available sequencing instrument. The NovaSeq 6000, acquired through an S10 grant in 2021, would greatly benefit from automated sample handling for optimal operation. Availability of these platforms has spurred an increase in sequencing demand that exceeds our manual handling capabilities. Acquisition of the Hamilton NGS STAR platform will ensure that laboratories supported by this facility, especially the NIH-funded investigators described herein, as well as young investigators who rely on MetaOmics Shared Resource resources for their first NIH grant proposals, will continue to receive timely and cost-effective service. The Hamilton NGS STAR works in conjunction with Illumina Sequencing Systems to offer higher precision, reproducibility, increased sample capacity, faster turnaround and more cost-effective sequencing. Increased efficiency and cost savings will support and expedite research in genetic diseases, complex traits, and mechanisms of tumorigenesis, metastasis, and therapeutic response. Strengths of this proposal include: support from NCI-designated SKCC and TJU administration; leadership experience and biotechnology expertise of the PI and key personnel; the large-scale infrastructure of the TJU academic, clinical, and innovation pillars; highly productive faculty with innovative research projects. Acquisition of this equipment will have long-lasting impact on biomedical research at TJU.