Whole genome sequencing laboratory, procurement of supplies and reagents and equipment maintenance for surveillance, outbreak assessment, and data mining.

摘要： 路易斯安那州动物疾病诊断实验室（LANDO）是四个全基因组 Vet-LIRN选择的测序实验室参与试点项目， 全基因组测序（WGS）监测人畜共患病的耐药性 病原体该提案的长期目标是增加来自动物的基因组数据， 动物饲料细菌分离株到国家抗菌药物耐药性监测系统 并建立快速反应网络，以准确解决假定的动物性食品- 传播疾病的爆发。该项目有三个具体目标：1。提供资金 继续进行Vet-LIRN试验的用品、试剂和设备维护 抗生素耐药性（AMR）和测序项目。2.通过全基因组 测序：a）监测动物饲料和动物饲料中的抗菌素耐药性， 受感染的动物; B）建立参考数据库，以帮助解决食源性疫情 c）通过将食物来源和食物来源联系起来， 疾病的集群。3.为诊断病例或病原体提供支持和检测 CVM-Vet-LIRN转介的兴趣。 抗生素耐药性是人类和动物面临的持续且日益严重的威胁 健康全基因组测序是一种最新的和更强大的工具， 研究它并监测它的发展和进展。测序也显示 在食源性疾病暴发的流行病学调查中具有很好的实用性。

Summary: Louisiana Animal Disease Diagnostic Laboratory (LADDL) is one of four whole genome sequencing laboratories selected by Vet-LIRN to participate in a pilot project using Whole Genome Sequencing (WGS) to monitor antimicrobial resistance in zoonotic pathogens. Long term goals of this proposal are to add genomic data from animal or animal feed bacterial isolates to National Antimicrobial Resistance Monitoring System and to develop a rapid response network for accurate resolution of putative animal food- borne disease outbreaks. This project has 3 specific aims: 1. Provide funding for supplies, reagents and equipment maintenance for continuation of Vet-LIRN’s pilot Antimicrobial Resistance (AMR) and Sequencing Project. 2. Through whole genome sequencing: a) provide surveillance of antimicrobial resistance in animal feeds and in infected animals; b) build reference databases to aid in solving foodborne outbreaks more efficiently; and c) aid foodborne disease investigations by linking food sources and clusters of disease. 3. Provide support and testing for diagnostic cases or pathogens of interest referred by CVM-Vet-LIRN. Antimicrobial resistance is a constant and growing threat to both human and animal health. Whole genome sequencing is one of the recent and more powerful tools to study it and monitor its development and progression. Sequencing also has shown excellent utility in the epidemiological investigations of food-borne disease outbreaks.