JAK-STAT Control of Zika Virus-Induced Fetal Injury

项目摘要 目前的项目“控制寨卡病毒引起的胎儿损伤”(R01AI143265)，包括 许多非人灵长类(NHP)和人类组织和细胞样本的加工 吞吐量RNA测序(RNAseq)分析。我们的样品处理流水线 检测方法包括RNA提取和质控(QC)、文库构建和质控。 我们目前使用Biomek FX自动化工作站来执行这些工作的大部分 在我们用于这些分析的高吞吐量工作负载中处理大容量样品的步骤。这个 BIOMEK FX成立10年了。由于年龄的原因，它不可靠，有能力获得未来的服务 合同不能保证，当前配置和软件与OFF-2不兼容 -搁置协议。因此，我们需要资金来取代这一工具。购买一辆 BIOMEK i7混合自动化工作站取代了我们的Biomek FX工作站，使我们能够 实现我们规定的项目目标。 0

Project Summary The current project, "Jak STAT control of Zika virus-induced fetal injury" (R01 AI143265), includes the processing of many nonhuman primate (NHP) and human tissue and cell samples for high throughput RNA sequencing (RNAseq) analysis. Our sample-processing pipeline for these assays includes RNA isolation and quality control (QC), and library construction and library QC. We currently utilize a Biomek FX automated workstation to perform the majority of these work steps in handling high volume samples in our high throughput workload for these analyses. The Biomek FX is 10 years old. Due to the age, it is not reliable, the ability to obtain a future service contract is not guaranteed, and the current configuration and software are not compatible with off- the-shelf protocols. Thus, we are in need of funds to replace this instrument. The purchase of a Biomek i7 Hybrid automated workstation to replace our Biomek FX workstation, allows us to achieve our stated project goals. 0