Critical Resources Provided by UNMC RAP Biorepository Stimulate Cancer Research

项目摘要 高度创新的快速尸检程序（RAP）解决了胰腺和前列腺的主要局限性 癌症研究：缺乏代表疾病所有阶段的高质量癌症人体标本。这 项目由保罗·M博士领导。Grandgenett，包括快速收集大量的所有器官， 在死亡后5小时内补充液体，是世界上独一无二的计划，旨在维持细胞和 组织的分子完整性RAP是内布拉斯加大学医学中心的核心组成部分 （UNMC）胰腺癌研究计划和GU肿瘤焦点小组，其任务是 收集、存档和分发来自最近因胰腺炎去世的患者的组织和液体 或者前列腺癌。弗雷德和帕梅拉胰腺癌研究项目的单位主任 巴菲特癌症中心是迈克尔·A·霍林斯沃思，而前列腺组是由博士领导的巴特拉和 博士肖娜波义耳。Grandgenett博士是Hollingsworth博士支持下的研究副教授， RAP的主任和这个研究专家奖的申请人。快速尸检程序 在UNMC胰腺癌研究计划中，它对以下方面很重要，目前部分由以下方面资助： 胰腺癌SPORE p50补助金;正在进行和即将进行的P01和U 01赠款以及几项R 01赠款， 个别调查员。Grandgenett博士亲自指导收集原发性肿瘤，残留未受影响的 组织、远处转移、所有未受影响的组织，以及所有PDAC的液体如血液和腹水， 前列腺癌患者。他还建立了一个广泛的正常组织采集计划， 利用。Grandgenett博士根据以下需求提前确定特定样本的优先顺序： 在联合国监测委员会和其他机构资助的项目。目前，Grandgenett博士提供新鲜样本， 多个实验室，在UNMC和外部UNMC，进行基于血液的生物标志物（蛋白质， RNA、DNA）、循环肿瘤细胞、包括类器官和异种移植物的患者来源的建模，以及其他 流程.将剩余样品快速冷冻，长期储存并提供 通过当地、国家和国际癌症研究的请求和合作。博士的主要角色。 Grandgenett将协调30名RAP志愿者的培训和参与，并与 合作者确定其研究项目所需的样本，根据需要修改我们的SOP， 获取这些样本，确认样本质量，然后将这些样本分发给研究者。是 这一资源的领导是一个训练有素的研究专家，具有广泛的 通过参与尸检获得经验，全面了解患者样本可用性， 以及在科学层面上与每位研究人员互动的能力。葛兰杰奈特博士在 这些生物标本的收集、加工、注释和分发的所有方面， 在扩大与联合国监测委员会调查员的合作项目方面以及在国家和国际一级发挥作用。

Project Summary The highly innovative Rapid Autopsy Program (RAP) addresses a major limitation in pancreatic and prostate cancer research: the lack of quality human specimens of cancer representing all stages of disease. This program, led by Dr. Paul M. Grandgenett, which includes rapid collection of large quantities of all organs and fluids within 5 hours of death, is a unique program in the world and is designed to maintain cellular and molecular integrity of the tissue. The RAP is a core component of the University of Nebraska Medical Center (UNMC) Pancreatic Cancer Research program and the GU Oncology Focus Group, that is tasked with the collection, archiving and distribution of tissues and fluids from patients that have recently passed with pancreas or prostate cancer. The Unit Director of the Pancreatic Cancer Research program at the Fred and Pamela Buffett Cancer Center is Dr. Michael A. Hollingsworth, while the prostate group is led by Dr. Surinder Batra and Dr. Shawna Boyle. Dr. Grandgenett, a Research Associate Professor under Dr. Hollingsworth's aegis, is the director of the RAP and the applicant for this Research Specialist Award. The rapid autopsy program operates within the Pancreatic Cancer Research Program at UNMC and is important to and currently funded in part by: a pancreatic cancer SPORE p50 grant; ongoing and pending P01 and U01 grants and several R01 grants to individual investigators. Dr. Grandgenett personally directs the collection of primary tumor, residual unaffected tissues, distant metastases, all unaffected tissues, as well as fluids such as blood and ascites for all PDAC and Prostate cancer patients. He has also established an extensive normal tissue acquisition program that is highly utilized. Dr. Grandgenett determines in advance how specific samples will be prioritized based on the needs of funded projects both at UNMC and at other institutions. Currently, Dr. Grandgenett provides fresh samples to multiple laboratories, at UNMC and outside-UNMC, conducting research on blood-based biomarkers (proteins, RNA, DNA), circulating tumor cells, patient-derived modeling including organoids and xenografts, and other processes. The remaining samples are snap frozen, maintained in long-term storage and made available through request and collaboration for local, national and international cancer studies. Key roles of Dr. Grandgenett is to coordinate the training and participation of the thirty RAP volunteers and to work with collaborators to determine the samples necessary for their research project, to modify our SOP as needed to obtain those samples, confirm specimen quality and then to distribute those samples to investigators. It is essential for the leadership of this resource to be a highly trained Research Specialist with extensive experience through participation in the autopsies, a comprehensive knowledge of patient sample availability, as well as the ability to interact on a scientific level with each investigator. Dr. Grandgenett has a major role in all aspects of the collection, processing, annotation and distribution of these biospecimens and plays a vital role in expanding collaborative projects with investigators at UNMC and on a national and international level.