BioRepository and Precision Pathology Center

摘要-生物库与精准病理中心共享资源 生物信息库和精密病理中心(BRPC)提供基于患者组织和血液的研究 对杜克癌症研究所(DCI)的支持。主要服务领域是生物银行(BRPC广泛同意、组织、 血液和液体样本的采购、加工、储存和分发，包括为BRPC的患者- 衍生异种移植(PDX)和原代细胞培养(PCC)服务线，通过 宏观/微观解剖、DNA/RNA提取)、监管(方案审查、工作创建范围、预算编制、 如果可能，可广泛同意整合)和免疫组织学(传统组织学，单一和多重 免疫组织化学、原位杂交、组织微阵列创建、全切片成像和图像分析)。 关于生物库服务，BRPC对标本执行杜克大学最大的广泛同意协议 收集研究，这正在成为新的微生物组倡议的中坚力量，并已 自2012年以来，同意接受治疗的患者有4700人。在这些患者中，17%是非裔美国人，4%是其他非 高加索人，支持对服务不足的少数民族癌症的研究。BRPC还运营着一所 美国病理学家(CAP)认可的正常和疾病样本的生物信息库，代表一系列 固体和血液淋巴病理。银行活动与抽血、标准- 护理、活组织检查和外科手术以及临床试验支持的组织采集。BRPC确定活动的优先顺序 根据DCI疾病现场小组组长的指示。通常，单个生物库事件可以同时 提供多项正在进行的研究工作，准备PDX模型，准备PCCs，并为未来储存生物库 需要。BRPC生物样品也被用于NCI的CPTAC项目第三阶段。BRPC还提供 作为DCI个性化癌症医学倡议(PCMI)的行政和监管骨干， 它将基因组信息、临床注释和生物标本联系起来。通往石蜡组织的大门 BRPC病理科的档案提供监管支持、队列识别和 病理学家回顾使用这些档案进行的回顾性研究。BRPC快速检索和准备患者的 提交给临床试验赞助商的石蜡档案组织样本。最后，BRPC提供了专门的 组织加工和实验服务，包括基础组织学、动物的兽医病理学支持 模型，免疫组织化学，原位杂交分析，组织微阵列创建，肿瘤浓缩 显微解剖和激光捕获显微解剖、全幻灯片成像和图像分析算法。BRPC 为癌症基因组图谱项目作出贡献的生物谱系支持了许多高影响力的 发表在《细胞》、《自然》、《癌症细胞》和《细胞报告》上。 2018年，BRPC为177名调查人员提供了服务，其中58%是DCI成员，他们 占总使用量的92%，代表了所有8个DCI Research项目。DCI成员使用BRPC， 在本项目期间发表了170篇文章，其中54篇发表在影响较大的期刊上。

ABSTRACT – BIOREPOSITORY & PRECISION PATHOLOGY CENTER SHARED RESOURCE The BioRepository & Precision Pathology Center (BRPC) provides patient tissue and blood-based research support for the Duke Cancer Institute (DCI). Major service areas are Biobanking (BRPC broad consent, tissue, blood and fluid sample procurement, processing, storage and distribution including for the BRPC’s patient- derived xenograft (PDX) and primary cell culture (PCC) service lines, tumor enrichment through macro/microdissection, DNA/RNA extraction), Regulatory (protocol review, scope of work creation, budgeting, integration with broad consent if possible), and ImmunoHistology (traditional histology, single and multiplexed immunohistochemistry, in situ hybridization, tissue microarray creation, whole slide imaging and image analysis). Regarding the Biobanking service, the BRPC administers Duke’s largest broad consent protocol for specimen collection for research, which is becoming the backbone consent for new microbiome initiatives and has consented >4,700 patients since 2012. Of these patients, 17% are African American and 4% other non- Caucasian, supporting research into cancers of underserved minorities. The BRPC also operates a College of American Pathologists’ (CAP)-accredited biorepository of normal and diseased samples representing a range of solid and hematolymphoid pathologies. Banking activities occur in conjunction with blood draws, standard-of- care biopsy and surgical procedures and clinical trial-supported tissue acquisitions. BRPC prioritizes activities based on instructions of DCI’s disease-site group leaders. Often a single biobanking event can simultaneously supply multiple ongoing research efforts, prepare a PDX model, prepare PCCs, and stock the biobank for future needs. BRPC biospecimens also are being used in Phase III of the NCI’s CPTAC project. The BRPC also serves as the administrative and regulatory backbone for the DCI’s Personalized Cancer Medicine Initiative (PCMI), which links genomic information, clinical annotation, and biospecimens. The gateway to the paraffin tissue archives in the Department of Pathology, BRPC provides regulatory support, cohort identification, and pathologist review for retrospective studies using those archives. BRPC rapidly retrieves and prepares patients’ paraffin archival tissue samples for submission to clinical trial sponsors. Finally, the BRPC provides specialized tissue processing and experimental services including basic histology, veterinary pathology support for animal models, immunohistochemistry, in situ hybridization assays, tissue microarray creation, tumor enrichment by microdissection and laser capture microdissection, whole slide imaging and image analysis algorithms. BRPC biospecimens contributed to the Cancer Genome Atlas Project have supported numerous high-impact publications in Cell, Nature, Cancer Cell, and Cell Reports. In 2018, the BRPC provided services to 177 investigators, 58% of whom were DCI members who accounted for 92% of total usage and represented all 8 DCI Research Programs. Use of BRPC by DCI Members, contributed to 170 publications over the current project period, of which 54 were in high impact journals.