The creation of a digital shareable archive of high-resolution images of the human temporal bone tissue

摘要 在U24拨款1U24DC015910用于NIDCD国家颞骨的本补编中 在加州大学洛杉矶分校的实验室，我们的目标是提供人类内耳组织病理学图像，用于教育和 科学目的。我们U24的这个附录的具体目的是创建一个高分辨率的数字 NIDCD国家颞骨标本档案。将共享颞骨H&E数字化图像 到NIDCD登记处和数据库。加州大学洛杉矶分校的NIDCD国家颞骨实验室拥有 美国最大的档案颞骨收藏(约1200对)。在U24人类之下 在颞骨赠款方面，该实验室积极致力于保存和获取颞骨 并与许多基础科学家和临床科学家合作。我们已经达到了 U24家长资助：1)改善听觉和前庭研究社区接触人类内耳的机会 组织2)组织准备技术的优化3)可视化成像技术的发展 人类中耳和内耳和4)住院医生和科学家在人类颞叶研究中的培训 骨组织病理学。加州大学洛杉矶分校存档的人类颞骨标本是独一无二的，因为我们的许多人 标本是在最短的死后时间内获得的，允许极好的形态保存。在……里面 为了传播人类颞骨组织病理学作为一种教育工具和手段 与科学家合作，我们提交了对NIDCD国家时态报告进行补充的请求 加州大学洛杉矶分校的骨实验室为创建高分辨率数字图书馆提供了专门的设备。 使用火棉胶包埋的人类颞骨有助于驱动基本的动物内耳 对人类耳部病理的认识和研究具有很高的发展潜力 恢复听觉和前庭功能的有效药物疗法。本建议书中产生的材料 在听觉和前庭研究方面进行动物研究的艾滋病研究科学家 在人类身上验证他们的发现。附录中要求的成像系统将允许创建 苏木素和伊红切片的高分辨率数字图像，所有的颞骨都可以在我们的 实验室。这些数字图像将被上传到国家颞骨登记和数据库。

ABSTRACT In the present supplement to the U24 grant 1U24DC015910 for the NIDCD National Temporal Bone Laboratory at UCLA, we aim to make available human inner ear histopathological images for educational and scientific purposes. The specific aim of this supplement to our U24 is the creation of a high-resolution digital archive of the NIDCD National Temporal Bone specimens. Temporal bone H&E digitized images will be shared to the NIDCD Registry and Database. The NIDCD National Temporal Bone Laboratory at UCLA houses one of the largest collections of archival temporal bones (approximately 1200 pairs) in USA. Under the U24 Human Temporal Bone grant, the Laboratory has actively pursued the preservation and acquisition of temporal bones and has collaborated with many basic scientists and clinician scientists. We have met the specific aims of the U24 parent grant: 1) to improve access for the auditory and vestibular research community to human inner ear tissues 2) Optimization of tissue preparation techniques 3) the development of imaging techniques to visualize human middle and inner ear and 4) the training of residents and scientists in the study of the human temporal bone histopathology. The UCLA archival human temporal bone specimens are unique since many of our specimens were obtained with minimal postmortem time allowing for superb morphological preservation. In order to disseminate the human temporal bone histopathology as an educational tool, and as a means of collaboration with scientists, we submit the present request for supplement to the NIDCD National Temporal Bone Laboratory at UCLA for specialized equipment to allow for the creation of high-resolution digital library. The use of celloidin-embedded human temporal bones has been instrumental in driving basic animal inner ear research into the understanding of human ear pathology and has a high potential for the development of effective drug therapies for restoring auditory and vestibular function. The materials generated in this proposal aids research scientists who are conducting animal studies in auditory and vestibular research by providing validation of their findings in the human. The imaging system requested in the supplement will allow to create a high-resolution digital image of hematoxylin and eosin sections of all the temporal bones available in our laboratory. These digital images will be uploaded to the National Temporal Bone registry and database.