Flow Cytometry

项目摘要/摘要 Tisch癌症研究所(TCI)流式细胞术共享资源设施(FCSRF)为TCI成员提供 能够获得最先进的技术和专业知识，以成功开展科学研究 项目。流式细胞术是一项强大的技术，使研究人员能够同时研究 破译复杂细胞和分子的每秒数千个细胞的多参数特性 健康和疾病中的过程。FCSRF由机构流式细胞术核心和两个TCI组成 卫星站点。FCSRF配备了六个分析仪和六个细胞分类器，包含在生物安全级别2+ 专门用于分离感染II类病原体的非固定人类样本和小鼠细胞的区域。在……里面 此外，两个卫星站点配备了四台分析仪。配备了7名流式细胞仪专家 SRF 100%致力于该设施，提供运营、培训和咨询服务，涵盖所有 流式细胞术的一些方面，包括基本细胞生物学原理，分析，分离单个细胞 癌症患者，并鉴定同一细胞上30多种不同的细胞表面/细胞内抗原 使用革命性的极光平台。FCSRF还为基础应用提供服务，例如 单细胞分离，以支持涉及RNA-SEQ、CHIP-SEQ、ATAC-SEQ的创新和复杂的生物学研究 SEQ，并与蛋白质组学、代谢组学和脂质组学图谱一起引用-SEQ。考虑到这一点，我们的 任务是提供有关流式细胞仪分析和细胞的必要仪器和专业知识 分类，以便TCI实验室通过基础知识提高人类癌症知识、诊断和治疗 科学、翻译和临床研究。

PROJECT SUMMARY/ABSTRACT The Tisch Cancer Institute (TCI) Flow Cytometry Shared Resource Facility (FCSRF) provides TCI members with access to state-of-the-art technology and expertise for the successful development of scientific research projects. Flow cytometry is a powerful technology that enables researchers to study simultaneous multiparametric characteristics of thousands of cells per second to decipher complex cellular and molecular processes in health and disease. The FCSRF comprises the institutional Flow Cytometry Core and two TCI satellite sites. The FCSRF is equipped with six analyzers and six cell sorters contained in biosafety level 2+ areas dedicated to isolate non-fixed human samples and mouse cells infected with class II pathogens. In addition, two satellite sites are equipped with four analyzers. Staffed with seven flow cytometry specialists committed at 100% to this facility, the SRF provides operational, training, and consultation services covering all aspects of flow cytometry including fundamental cell biology principles, analyses, isolation single cells from cancer patients, and identification of over thirty different cell surface/intracellular antigens on the same cell using the revolutionary Aurora platform. The FCSRF also offers services for fundamental applications, such as single cell isolation, to support innovative and complex biological studies involving RNA-seq, ChIP-seq, ATAC- seq, and CITE-seq together with proteomics, metabolomics, and lipidomics profiling. With this in mind, our mission is to provide the necessary instrumentation and expertise regarding flow cytometry analyses and cell sorting so that TCI laboratories improve human cancer knowledge, diagnosis, and treatment through basic sciences, translational, and clinical research.