NEURAL CELL ENGINEERING AND IMAGING CORE

项目摘要/摘要-(核心D：NCEI核心) 神经细胞工程和成像核心(Core D，NCEI)已经成为肯尼迪 中心30年，资源和用户基础稳步增长，拥有来自50个IDDRC的70人 仅在过去3年内使用核心的实验室。NCEI核心由3个主要部门组成：细胞 和组织工程子核心，拥有细胞/组织培养工作的设施和专业知识，包括原代， 胚胎、诱导性多能性细胞和现代神经分化方案。现代显微镜次核心，具有 用于组织切片和成像的仪器。和分析和图形子核心，它提供 配备复杂软件的工作站，可用于反卷积、定量2D到5D图像分析和 重建、定制软件和宏，以及图形和打印服务。优化使用 这些工具为IDDRC调查人员及其受训人员、NCEI领导层提供深入的用户咨询， 强调有计划的实验设计，并对项目进度进行警惕的监测。这个 CORE的价值在于扩展了与专家顾问的联系，允许进行基因靶向、基因治疗、RNA等研究 结构、光遗传学、电生理学，以及人类胚胎干细胞、诱导性多能性和胎儿细胞，包括有机体。 生产力和创新也通过与其他IDDRC核心的互动来提高，协调，例如， 脑细胞和解剖学变化与行为表型(AP)、遗传或表观遗传的相关性研究 分离细胞亚群/单细胞(NGEN)的评价和重编程的翻译研究 来自患者的细胞和IPSC衍生的有机类物质(HCP)。核心资源还为P50研究项目提供服务 KDM5C疾病通过提供基因表达变化的成像和定量分析，分析 小鼠模型中神经亚型和树突的变化，以及产生iPS细胞/器官和 从携带KDM5C突变的患者样本中区分神经元。核心保持了高标准的 通过计划的内部评估和由ADM进行的年度用户调查进行质量控制 核心。总的来说，NCEI的核心是遗传学和遗传学之间的科学桥梁的重要组成部分 认知表型和认知表型对IDDRC进行的研究具有广泛和重大的影响 研究人员和他们的受训人员专注于对IDDS的理解和治疗，并对其至关重要。

PROJECT SUMMARY/ABSTRACT – (Core D: NCEI Core) The Neural Cell Engineering and Imaging Core (Core D, NCEI) has been a vital component of the Kennedy Center for 30 years, steadily growing in resources and user base, with 70 individuals from 50 IDDRC laboratories utilizing the Core in the last 3 years alone. The NCEI Core consists of 3 major divisions: The Cell and Tissue Engineering Subcore, with facilities and expertise for cell/tissue culture work including primary, embryonic, iPS cells and modern neural differentiation protocols. The Modern Microscopy Subcore, with instruments for tissue sectioning and imaging. And the Analysis and Graphics Subcore, which provides workstations with sophisticated software for deconvolution, quantitative 2D to 5D image analyses and reconstruction, custom software and macros, as well as graphics and printing services. To optimize use of these tools for IDDRC investigators and their trainees, NCEI leadership provides in-depth user consultation, emphasizes well-planned experimental design, and engages in vigilant monitoring of project progress. The Core’s value is extended linkage with expert advisors allowing studies on gene targeting, gene therapy, RNA constructs, optogenetics, electrophysiology, and human ES, iPS and fetal cells, including organoids. Productivity and innovation are also enhanced by interaction with other IDDRC Cores, coordinating, e.g., studies correlating brain cell and anatomic changes with behavioral phenotypes (AP), genetic or epigenetic evaluations on isolated cell subpopulations/single cells (NGEN), and translational studies on reprogrammed cells and iPSC-derived organoids from patients (HCP). Core resources also serve the P50 research project on KDM5C disease by providing imaging and quantitative analyses of changes in gene expression, analysis of neural subtypes and dendritic changes in mouse models, and generating iPS cells/organoids and differentiating neurons from patient samples carrying KDM5C mutations. The Core maintains high standards of quality control through scheduled internal evaluations and the annual user survey carried out by the ADM Core. Collectively, the NCEI Core represents a significant part of the scientific bridge between genetics and cognitive phenotypes and as such has a broad and significant impact on the research carried out by IDDRC investigators and their trainees focused on, and critical to, understanding and treating IDDs.