Human Skin Disease Resource-based Center

项目摘要/摘要：总体组成部分 小鼠模型提供了许多生物学见解，并仍然是最受欢迎的进行实验的系统 皮肤病研究。然而。在皮肤和免疫系统之间有显著的不同 而老鼠和人类的这些差异是不完全的，这使得我们很难知道 在老鼠身上所做的观察也适用于人类。对人类细胞和组织进行的研究可以 解决这一知识鸿沟。人类生物库和强大的新分析技术已经成为可能 这使得高质量的人类皮肤病研究变得容易获得。该中心的目标是加速 人类皮肤病研究通过向任何机构的研究人员提供人类样本和 尖端分析技术，并将新的不同的研究人员带入人类皮肤病领域 研究。我们包括来自希望利用中心服务的研究人员的45个研究项目；然而，任何 希望开展人类皮肤病研究的研究人员是这项研究的潜在成员 社区。该中心由一个管理核心和三个资源核心组成。行政部门 CORE管理和监督中心的所有活动，并管理多样性和外联活动， 包括资助不同调查人员参加多个阶段的培训和两年一度的国际 人类皮肤病会议。人体组织生物库核心提供了超过11.3万个 高度特征化的同意患者，超过150万个保存的病理标本，两者都可以通过 诊断，以及新鲜的人皮肤，从人皮肤和免疫缺陷小鼠中纯化的细胞群 移植了人的皮肤和血液。单细胞和免疫分析核心提供对单细胞的访问 RNA和ATAC测序，基于流式细胞术的单细胞成像，飞行时间的质量细胞术 (CyTOF)和高通量TCR测序。下一代组织分析与成像核心 提供基于六色酪胺扩增的免疫染色结合光谱成像和 自动化细胞分析、纳米串RNA和DNA分析以及数字空间分析--最先进的技术 可以分析FFPE和冰冻切片中10-1000个蛋白质或RNA靶标表达的技术。 总而言之，该中心提供对生物库和尖端人类分析技术的访问，从而使 翻译研究人员开展高质量的人类皮肤病研究。

Project Summary/Abstract: Overall Component Mouse models have provided many biologic insights and remain the most popular system in which to conduct skin disease research. However. there are significant differences between the skin and immune systems of mice and humans and these differences are incompletely characterized, making it difficult to know if observations made in mice will hold true in humans. Research carried out on human cells and tissues can address this knowledge gap. Human biobanks and powerful new analytic techniques have become available that make high-quality human skin disease research accessible. The goals of this Center are to accelerate human skin disease research by providing researchers at any institution with access to human specimens and cutting edge analytic techniques and to bring new diverse investigators into the field of human skin disease research. We include 45 research projects from investigators who wish to utilize Center services; however, any researcher wishing to carry out human skin disease research is a potential member of the research community. The Center is composed of an Administrative Core and three Resource Cores. The Administrative Core manages and oversees all activities of the Center and administers Diversity and Outreach activities, including funding for diverse investigators at multiple stages of training and the biennial International Conference on Human Skin Disease. The Human Tissues Biobank Core provides access to over 113,000 highly characterized consented patients, over 1.5 million banked pathologic specimens, both searchable by diagnosis, as well as to fresh human skin, purified cell populations from human skin and immunodeficient mice grafted with human skin and blood. The Single Cell and Immunoanalysis Core provides access to single cell RNA and ATAC sequencing, flow cytometry-based single cell imaging, mass cytometry by time of flight (CyTOF) and high throughput TCR sequencing. The Next Generation Tissue Analysis & Imaging Core provides access to six color tyramide amplification based immunostaining combined with spectral imaging and automated cell analysis, NanoString RNA and DNA profiling and Digital Spatial Profiling, a state-of-the-art technique that that can profile expression of 10-1000s of protein or RNA targets in FFPE and frozen sections. In summary, the Center provides access to biobanks and cutting-edge human analytic techniques that enable translational researchers to carry out high quality human skin disease research.