Center for Structural Biology of HIV RNA

核心4：生物学和化学 总结 该核心组成部分的CRNA研究人员在病毒学、细胞和分子生物学方面拥有广泛的专业知识。 RNA生物学和化学。每一个以生物学为重点的项目都需要应用 这些领域的技术和专业知识。为促进确定结构和 生物物理分析，结构和生物物理数据的验证和生物解释，以及 鉴定铅小分子化学探针，生物和化学核心将承担一些 这些活动包括：（1）发现HIV-1 RNA和HIV-1 RNA-蛋白复合物的新特征， 使用下一代测序方法进行结构功能研究，（2）HyPR-MS、siRNA和 sCAS 9/SAM（CRISPR）筛选以识别和验证结构-功能研究的新蛋白质靶点，（3） 提供病毒学和一般分子生物学的支持，以生物和结构为重点的项目 和目的，和（4）在计算机上，在体外和细胞RNA靶向筛选和化学合成沿着 化学信息学/机器学习技术，使发现RNA靶向抑制剂的 在CRNA中研究的生物过程。

Core 4: Biology and Chemistry Summary CRNA investigators in this Core component have a wide range of expertise in virology, cell and molecular biology, RNA biology, and chemistry. Each of the biologically focused projects requires the application of techniques and expertise in these areas. To facilitate the identification of new targets for structural and biophysical analysis, the validation and biological interpretation of structural and biophysical data, and the identification of lead small molecule chemical probes, the Biology and Chemistry Core will undertake a number of activities including: (1) the discovery of new features of HIV-1 RNAs and HIV-1 RNA-protein complexes for structure function studies using next-generation sequencing-based approaches, (2) HyPR-MS, siRNA and sCAS9/SAM (CRISPR) screening to identify and validate new protein targets for structure-function studies, (3) the provision of virology and general molecular biology support to biologically and structurally focused projects and aims, and (4) in silico, in vitro and in cell RNA-targeted screening and chemical synthesis along with cheminformatics / machine learning techniques to enable the discovery of RNA-targeted inhibitors of the biological processes studied in the CRNA.